Introduction The construction and transfection of eukaryotic expression plasmid are commonly molecular biology method used in medical research.
序言真核表达质粒的构建和转染是目前医学研究中常用的分子生物学手段。
The sequence of the cloned SCF is same as the published report. The construction of expression plasmid is identified in a correct way.
序列分析表明所克隆的SCF序列与文献报道的一致，构建的表达载体经鉴定正确。
The discussion was mainly focused on the application of these detection methods, the problem result in the virus extraction progress, and the construction of expression plasmid having LSV CP gene.
重点讨论了各种检测方法在实际中的应用，百合无症病毒提取过程中需要解决的问题，外壳蛋白基因在大肠杆菌中表达载体的构建。
Conclusions a clone of murine GAD65 is successfully constructed. It is a basis step for further construction of expression plasmid that expresses GAD65 and for immunologic intervention of T1DM.
结论成功的克隆了小鼠gad65基因，为构建GAD65基因疫苗以及糖尿病的免疫干预治疗打下基础。
Conclusions a clone of murine GAD65 is successfully constructed. It is a basis step for further construction of expression plasmid that expresses GAD65 and for immunologic intervention of T1DM.
结论成功的克隆了小鼠gad65基因，为构建GAD65基因疫苗以及糖尿病的免疫干预治疗打下基础。
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