Objective: To study the expression of murine stem cell factor in CHO cells.
目的:克隆造血干细胞因子并在CHO细胞中表达。
AIM: TO obtain high level expression of recombinant human truncated osteoprotegerin (TOPG) with higher bioactivity in CHO-DHFR-cells.
实现其在CHO - DHFR -细胞中的高效表达,获取生物活性较高的重组蛋白。
CONCLUSION: MTX induced-increase of recombinant antibody production in CHO cells can be increased by weakening the expression of DHFR gene.
结论:弱化表达载体中DHFR的表达,可提高MTX对工程抗体表达的增加效果。
Conclusion: MTX selection should be timely used to overcome the instability of the expression of anti HBsAg in CHO HB2 cells.
结论:应及时用MTX对细胞株进行再加压筛选以及保存,以保持细胞稳定表达抗体的能力;
Conclusion: MTX selection should be timely used to overcome the instability of the expression of anti HBsAg in CHO HB2 cells.
结论:应及时用MTX对细胞株进行再加压筛选以及保存,以保持细胞稳定表达抗体的能力;
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