The totipotent and chromosome of the expanded ES cells were also examined.
对扩增后的ES细胞进行染色体和全能性检测。
Feeder system was superior to feeder-free system during the culture of ES cells.
在ES细胞培养过程中，有饲养层体系优于无饲养层体系。
The methods for propagating the embryonic stem cells (ES cells) were studied in vitro.
探讨了胚胎干细胞在体外大量扩增的方法。
Therefore, es cells are the ideal model of development of cardiomyocytes and its key genes.
因此，ES细胞是研究心肌细胞发育分化机制及鉴定其关键基因的理想模型。
One issue in using uniparental ES cells for tissue transplants is their durability and safety.
使用单系胚胎干细胞进行组织移植中的问题便是其耐用性和安全性。
Human embryonic fibroblast feeder played a positive role during the ES cells isolation procedure.
人胚胎成纤维细胞饲养层在ES细胞分离过程中具有较好的作用。
Under appropriate conditions, es cells can differentiate into all cell types including germ cells.
在适当的条件下，ES细胞能分化成各种细胞类型，包括生殖细胞。
On the other hand, it may also limit the ability of these es cells to fully replicate those types of tissues.
但另一方面，它也可能限制了这类ES细胞完全修复该类组织。
Dr DE Sousa said that the aim of the research was to generate es cells so their development could be studied.
德·索萨博士说，这次研究的目的就是产生ES细胞，这样就可研究它们的发育。
Thus, we can obtain the ES mouse completely from ES cells with the method of tetraploid embryo complementation.
利用四倍体胚胎补偿技术就可以得到完全由ES细胞发育而来的小鼠。
Due to their unique properties, es cells are used in diverse fields of biological research, particular in developmental biology.
胚胎干细胞具有的独特生物学特性使其被广泛应用于生物学研究的各个领域，特别是发育学。
To answer these questions, the researchers applied live-cell imaging to the earliest phase of dissociation in human and mouse ES cells.
为了解答这些问题，研究人员运用活细胞成像技术到人胚胎干细胞和鼠胚胎干细胞最初的分离阶段。
Those ES cells carrying the inactivated gene can then be isolated and introduced (by injection) into a pre-embryonic structure called the blastocyst.
那些携带灭活基因的胚胚胎干细胞经分离出来后注入称作胚泡的胚前组织中。
Conclusion: ES cells should be cultivated on MEF feeder layer or in BRL conditioned medium plus MEF feeder layer at the early stage after recovering.
结论：在复苏后早期阶段ES细胞培养中，不宜单独应用BRL条件培养基，须用MEF饲养层或合并应用BRL条件培养基和MEF饲养层。
Despite the advantages of using mouse es cells in the study of gene regulatory networks, further research must focus more directly on human stem cells.
除了在本研究中利用基因调控网络中的小鼠胚胎干细胞的优势外，深入研究必须更加直接地集中于人类干细胞。
Ten years after the discovery of human es cells, scientists are still working on standardizing procedures for coaxing pluripotent cells to become mature tissue.
在发现人类胚胎干细胞的十年之后，科学家们仍致力于研究将多能细胞“哄骗”成为成熟组织细胞的标准过程。
Undifferentiated es cells can form tumors, for instance, creating a risk of cancer if the cells are not all successfully differentiated before transplantation.
例如，未分化的胚胎干细胞可能形成肿瘤，如果在移植前细胞未全部成功分化，将可能造成癌症。
On the one hand, it may actually be good to have ES cells with a particular propensity for differentiation, because it may make it easier to get certain types of tissue.
他说：“一方面，它可能是有利的，有ES细胞具有特异分化功能，因为这使获得特定的组织类型的细胞更容易。
The discovery implies that culture conditions during es cell generation - which have yet to be identified - can influence the developmental properties of human es cells.
这一发现表明在ES细胞传代时的培养条件(已被认识到)能影响人类ES细胞的发育特征。
Reported in the journal cell Stem cell, these results provide a first ever comprehensive elucidation of mechanisms underlying dissociation-induced apoptosis in human es cells.
这是首次比较全面的揭示了细胞分离导致人细胞凋亡的机制，被刊登在干细胞杂志上。
CONCLUSION: a simple and economical method was established to induce mouse es cells cultured in vitro to differentiate into cardiomyocytes without using any chemical reagents.
结论：本法无需添加化学诱导剂，为一种简便、经济的诱导体外培养小鼠es细胞分化为心肌细胞的方法。
Purification of Milli-Q water by sub-boiling distilled and employment of qualified FBS could increase the AKP staining positive level and total clone formation rate of mouse ES cells.
Q水经亚沸处理能显著提高ES细胞保持未分化状态的能力，优质胎牛血清能提高ES的AKP阳性度和克隆形成率。
Purification of Milli-Q water by sub-boiling distilled and employment of qualified FBS could increase the AKP staining positive level and total clone formation rate of mouse ES cells.
Q水经亚沸处理能显著提高ES细胞保持未分化状态的能力，优质胎牛血清能提高ES的AKP阳性度和克隆形成率。
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