PCR products were digested with endonuclease and sequenced.
PCR产物经限制性内切酶消化、序列测定及分析。
Methods:DNA sequencing and restriction endonuclease reaction was used.
方法：采用DNA测序及限制性酶切反应方法。
The structure of vector was identified by endonuclease analyzing and base sequencing.
通过核酸内切酶酶切分析及测序鉴定载体结构。
After a restriction endonuclease cleavage, two expected smaller fragments were observed.
经限制性内切酶反应，获得两个预期大小的片段。
The core technology in TILLING is screening mutant library by using single-strand endonuclease.
TILLING技术的核心是使用单链核酸内切酶大规模筛选突变体库。
The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
Then, we analyzed the specificity of PCR primers through digesting PCR products with restriction endonuclease.
然后对扩增产物进行限制性酶切反应；
In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.
本研究利用多种组合的酶切分析，绘制了较为详细的酶切图谱。
Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the AGAR gel electrophoresis.
最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。
The products of endonuclease digestion were run on 8% non-denaturing polyacrylamide gel electrophoresis and detected by silver staining.
用8%非变性聚丙烯酰胺凝胶电泳将酶切产物分离，并用银染法显色。
Restriction enzyme: Protein (more specifically, an endonuclease) produced by bacteria that cleaves DNA at specific sites along its length.
限制性内切：由细菌产生的一种蛋白质，能在特定的地方切断去氧核糖核酸分子。收藏。
Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful.
结果酶切及测序显示对OSCP基因有义突变部分的纠正获得成功。
Results: the constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.
结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45 - 4b基因。
Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.
结果经pcr方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。
Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;
并对发现突变的基因进行分析。方法应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段；
Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.
方法用盐提取法提取人群中白细胞的DNA，以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。
Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.
方法用盐提取法提取人群中白细胞的DNA，以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。
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