The changes of the proliferating nuclear antigen expression and apoptosis of anterior pituitary cells were detected with immunohistochemistry and in situ end labeling method.
分别采用免疫组化方法和原位末端标记法检测垂体前叶细胞增殖核抗原的表达及凋亡的变化情况。
Methods: PCO model of rat was established. Ovaries were fixed and processed for ultrastructural observation. DNA fragments in apoptotic cells were detected by in situ DNA 3 end labeling method.
方法：以大鼠多囊卵巢为动物模型，采用电子显微镜及3-末端原位标记法观察了大鼠多囊卵巢颗粒细胞凋亡的情况。
Method Apoptosis was determined by the method of in situ Nick end labeling. Apoptotic index (AI) means the Numbers of apoptotic cells per 100 cells.
方法采用原位切口末端标记方法检测细胞凋亡，平均每100个细胞内的阳性细胞数为凋亡指数(AI)。
Terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) technique and immunohistochemistry method were applied to detect NPC tissue and normal nasopharyngeal tissue (NNPT).
采用脱氧核糖核酸末端转移酶介导的缺口末端标记技术（TUNEL）和免疫组化方法检测鼻咽低分化鳞状细胞癌和正常鼻咽黏膜组织。
In situ end-labeling method (POD) indicates:HL-60 cells apoptosis induced by Liu-Shen-Wan alcohol extraction have time-concentration dependency relation within certain ranges;
原位末端标记表明：六神丸乙醇提取剂诱导HL-60细胞凋亡在一定范围内呈时间-浓度依赖关系；
In situ end-labeling method (POD) indicates:HL-60 cells apoptosis induced by Liu-Shen-Wan alcohol extraction have time-concentration dependency relation within certain ranges;
原位末端标记表明：六神丸乙醇提取剂诱导HL-60细胞凋亡在一定范围内呈时间-浓度依赖关系；
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