Objective:To study the individual and joint effects of BDNF and NT-3on the sur-vival and growth of neurons from the spinal cord of embryonic rat.
目的：观察脑源性神经生长因子（BDNF）和神经营养因子-3（NT-3）单独及联合应用对体外培养胚胎大鼠脊髓神经元存活和生长发育的影响。
The survival ratio and the colony differentiation ratio of embryonic rat midbrain neurons were estimated by MTT assay and microscopy respectively.
采用MTT分析、形态学观察等方法，观察细胞存活率和集落分化率。
Objective to explore the culture conditions for the neural stem cells from embryonic rat cortices and to test their efficacy of expressing exogenous gene.
目的探讨胚胎大鼠神经干细胞的体外培养条件及外源基因的表达效率。
Objective to isolate neuroepithelial stem cells from the spinal cord neural tube of the embryonic rat and induce them to differentiate into dopaminergic neurons.
目的从胚胎大鼠脊髓神经管中分离神经上皮干细胞并诱导其向多巴胺能神经元方向分化。
Methods: Primary embryonic rat cortical neurons culture, cytotoxicity assays and biochemistry detection, cell stain for the neuron morphology in microscopy and image analysis.
方法：原代胚胎鼠皮质神经元细胞培养，细胞毒性试验及生化指标测定，细胞染色，显微镜下观察形态学改变及影像分析。
The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum.
方法从胚胎大鼠的大脑皮质、海马、纹状体等组织分离神经干细胞，用无血清培养技术在体外进行培养、扩增、传代和诱导分化。
Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord.
方法：利用无血清培养和细胞克隆培养技术，从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞，进行体外扩增培养、传代、贴壁分化观察。
Results: KNJ had no toxicologic effect in the general status, body weight change, and embryonic rat development, maternal rat delivery, newborn rat as well as the development status of rat.
结果：康乃精颗粒对大鼠的一般状况、体重变化和胎鼠发育、母鼠分娩、新生仔鼠以及子代大鼠的发育状况均无任何毒性作用。
Objective: to investigate the effects of Ginkgolide on the apoptosis of cultured neurons in the rat embryonic basal forebrain.
目的：探讨银杏内酯对体外培养的鼠胚基底前脑神经元凋亡的影响。
Rat embryonic fibroblasts (REF) can be used as feeder cells in cultivating rat embryonic stem cells.
大鼠胚胎成纤维细胞(REF)可以作为培养大鼠胚胎干细胞的滋养层。
Objective: to isolate and incubate neural progenitor cells from rat embryonic retina and to explore their characteristics of differentiation.
目的：建立大鼠胚胎视网膜祖细胞体外培养方法并观察其分化特性。
Objective To investigate the feasibility of treatment of focal cerebral ischemic-reperfusion injury with the rat embryonic neural stem cells (NSCs) transplantation in the rat models.
目的探讨大鼠胚胎神经干细胞移植治疗局灶性脑缺血再灌注损伤的可行性。
Objective:To observe long-term therapeutic effects of intracerebral transplantation of embryonic mesencephalic cells in a rat model of Parkinson's disease(PD).
目的：观察胚胎中脑细胞脑内移植治疗帕金森病（PD）模型大鼠的长期疗效。
Objective:To observe long-term therapeutic effects of intracerebral transplantation of embryonic mesencephalic cells in a rat model of Parkinson's disease(PD).
目的：观察胚胎中脑细胞脑内移植治疗帕金森病（PD）模型大鼠的长期疗效。
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