Established upon the embryo stem cell technique and homologous recombination, gene targeting has been widely used in the genome specific manipulation.
基因打靶技术是建立在胚胎干细胞和同源重组技术之上，可对基因组进行定点修饰的实验方法。
However, nuclear reprogramming, which stands in the way of reconstructed embryo stem cell research, is still a bottleneck in the procedure of nuclear transfer.
但是核重编程仍是核移植技术的瓶颈，制约了重构胚胎干细胞的研究。
Two research teams in the US and Japan have made embryonic stem cells – or something very like them – directly from human skin cells, without having to destroy an embryo in the process.
美国和日本的两个研究小组已直接从人体皮肤细胞中提取出胚胎干细胞（或非常类似的细胞），同时在这一过程中没有破坏胚胎。
In an E-mail, the journal cited the highly publicized 2004 fraud that came out of South Korea, where researchers led by Hwang woo-Suk claimed to have produced stem cells from a cloned human embryo.
在一封e - mail中，自然杂志特别引用了2004年广为传播的韩国Hwangwoo - Suk小组得到人类克隆胚胎的丑闻。
Scientists have found they can create chimeric animals that have organs belonging to another species by injecting stem cells into the embryo of another species.
科学家们发现只需向动物胚胎中注射另一物种的干细胞，他们便能创造出一种拥有另一物种器官的嵌合体动物。
Scientists have found that by injecting stem cells into the embryo of one species, they can create animals that have organs belonging to another species.
科学家们发现，向一种物种的胚胎中注射干细胞，这种物种就会长出另外一个物种的器官。
Researchers have succeeded in developing a human embryonic stem-cell line from an embryo that had died naturally.
研究者已经成功的从自然死亡的胚胎当中培育出了一个人胚胎干细胞系。
One rejected proposal included a plan to clone a human embryo and use it to produce new stem cells. Dr.
一项被拒绝的方案包括计划克隆一人胚胎、并用之来制造新的干细胞。
Stem cells are the source of all other cells. To get the cells someone has to take apart a human embryo.
干细胞即为起源细胞，需要从人类胚胎中提取。
If you believe that human life comes into existence as soon as the cells start to grow, then the practice of removing human stem cells from an embryo kills it.
如果你相信人类的生命随着细胞开始成长而存在，那么从胚胎中移除人体干细胞的行为就是杀人。
What we have done, for the first time, is to actually create human embryonic stem cells without destroying the embryo itself.
我们的工作首次实现了在获得人胚胎干细胞的同时不伤害胚胎本身。
The effect of feeder cell type, growth factors, and embryo developmental stage on establishment of sheep embryonic stem cell was also studied in present experiment.
同时，比较了饲养层细胞类型、生长因子、胚胎发育阶段等因素对绵羊胚胎干细胞分离培养效果的影响。
BACKGROUND: Presently, some problems still exists during establishment and culture of embryo multipotential stem cells, such as low rate of establishment, low rate of stem cells with normal karyotype.
背景：目前在胚胎多潜能干细胞建系和培养过程中，存在着建系率低、具有正常核型的干细胞系比率低等问题。
Methods human neural stem cells were separated from 10 ~ 13 weeks brains of human embryo and were cultured and induced to differentiate.
方法从自然流产的孕10 ~13周的人胚脑组织中分离、培养神经干细胞。
Objective To evaluate the characteristics of proliferation, migration and differentiation of neural stem cells in the brain of rat embryo during development.
目的观察胎鼠发育过程中脑内神经干细胞增殖、迁移和分化特点。
Methods the neural stem cells were isolated, identified and amplified from the cortex of aborted human embryo with 10 to 14-week gestational age, then telomerase activity was detected by PCR-ELISA.
方法分离、鉴定并扩增取自10 ~ 14周药物流产胎儿大脑皮质的神经干细胞，应用pcr - ELISA法检测神经干细胞端粒酶活性。
Up to now, human embryonic stem cells have been derived at a later stage of development when the embryo consists of about 150 cells.
到目前为止，人类胚胎干细胞都来自于发育比较晚的的阶段，那个时候胚胎大约有150个细胞。
Cancer patients from normal cell nucleus to replace the nucleus of human embryos, embryo transfer and the establishment of stem cell lines.
抽取癌症病人正常细胞的细胞核，替换人类受精卵细胞核，进行胚胎移植，建立干细胞系。
The International Stem Cell Corporation (ISCo), in Oceanside, California, for one, reckons it can do so and, at the same time, deal with the ethical issue of embryo destruction.
比如，坐落于加州欧申赛德的国际干细胞公司就是其中一个，该公司认为他们不仅能完成Geron所做的，并能同时处理好杀死胚胎方面的道德问题。
Objective In order to investigate whether the 12-week-old embryo brain choroid plexus epithelial cells have neural stem cell biological properties.
目的探讨人12周龄胚胎脑脉络丛上皮细胞是否具有神经干细胞的生物学特性。
Objective In order to investigate whether the 12-week-old embryo brain choroid plexus epithelial cells have neural stem cell biological properties.
目的探讨人12周龄胚胎脑脉络丛上皮细胞是否具有神经干细胞的生物学特性。
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