MethodsChromatographic fingerprint of Semen Cassiae was investigated by RP-HPLC and the gradient elution mode was applied to chromatographic separation.
方法采用RP -HPLC法，梯度洗脱，研究决明子饮片的指纹图谱，并作相似度比较分析。
Methods: The gradient elution mode was applied in chromatographic separation and data were analyzed to develop HPLC fingerprint analysis of Safflor Yellow.
方法：采用梯度洗脱的方法进行色谱分离，对各色谱峰进行数据处理，得出红花黄色素的指纹图谱。
The elution mode, composition of the mobile phase, content of the organic acid and the flow-rate of the mobile phase were optimized and thus a new flow rate gradient was proposed.
对制备色谱的洗脱方式、洗脱剂组成、浓度、洗脱速度等参数进行优化，提出并应用了流动相速度梯度洗脱方法。
The elution mode, composition of the mobile phase, content of the organic acid and the flow-rate of the mobile phase were optimized and thus a new flow rate gradient was proposed.
对制备色谱的洗脱方式、洗脱剂组成、浓度、洗脱速度等参数进行优化，提出并应用了流动相速度梯度洗脱方法。
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