The recombinant Bacmid containing fused ORF220 and EGFP gene was confirmed by PCR.
通过PCR确认了含有融合的ORF220和EGFP基因的重组芽孢杆菌酰胺。
Results CLLEGFP can infect various human derived cell lines and express EGFP.
结果CLLEGFP能够感染各种人源细胞并表达EGFP。
But wild type GFP and EGFP are not ideal to detect the transient changes of gene expression regulation.
但野生型GFP及增强型GFP等因半衰期长，稳定性强，对反映一过性转录表达调控的变化不甚理想。
The recombinant Bacmid containing fused ORF220 and EGFP gene was confirmed by PCR and then transfected into Sf21 cells.
用PCR鉴定含有ORF220和EGFP基因的重组质粒，提取纯化重组质粒并转染昆虫细胞进行表达。
After 2 weeks, the EGFP expression in the rats' retina and choroids were detected by confocal laser scanning microscopy.
转染2周后，在激光共聚焦显微镜下观察egfp表达情况。
Also, it seems that a time course of expression was important in the analysis although no quantitation was provided for the EGFP or the VEGF.
此外，它似乎一种表达的时间当然是在分析的重要，虽然没有定量的EGFP或血管内皮生长因子。
Results The full-length coding sequence of IDO was cloned from activated human leukocytes and the expression vector for IDO-EGFP fusion protein was constructed.
结果从活化的人白细胞中克隆到IDO基因编码区全长，并构建了IDOEGFP融合蛋白表达载体。
Objective:To establish a stable CHO cell line expressing GLUT4-EGFP, and to lay a foundation for investigating the translocation mechanisms of GLUT4 in CHO cells.
目的：建立稳定表达EGFP标记的葡萄糖转运蛋白4的CHO细胞系，为研究GLUT4在CHO细胞中的转运调节机制奠定基础。
RESULTS: DDR2/EGFP fusion plasmid was successfully constructed. Further analysis also demonstrated that the fusion protein has similar expression and activation pattern with wild type ones.
结果：成功构建了DDR2/EGFP融合表达载体，进一步的分析也证明此融合表达载体能在细胞中正确表达并可以被配体所激活。
Objective To construct the retroviral(RV) vector with report gene enhanced green fluorescent protein(EGFP) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells.
目的构建携带报告基因增强型绿色荧光蛋白（EGFP）的反转录病毒载体，并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。
Objective To construct the retroviral(RV) vector with report gene enhanced green fluorescent protein(EGFP) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells.
目的构建携带报告基因增强型绿色荧光蛋白（EGFP）的反转录病毒载体，并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。
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