Method: Establishing the EAE model and detecting the expression and distribution of MMP-2 and -9 in different type EAE with the method of IHC.

方法：建立多病程大鼠eae模型，以免疫组化的方法检测MMP - 2、MMP -9在不同类型EAE中的表达及分布。

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Methods Guineapigs were induced to establish EAE model by injecting guineapig with spinal cord homogenate (GPSCH) in complete Freund's adjuvant (CFA) bordetella pertussis vaccine (BPV).

方法采用注射完全福（氏）佐剂-豚鼠全脊髓匀浆（CFA-GPSCH） ，并辅以注射百日咳疫苗（ BPV） ，诱导豚鼠EAE模型。

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Objective:To establish the experimental autoimmune encephalomyelitis(EAE) model on Guinea Pig with pan-spinal cord homogenate(coarse myelin basic protein, cMBP).

目的：用全脊髓匀浆（粗制髓鞘碱性蛋白）建立豚鼠实验性自身免疫性脑脊髓炎（EAE）模型。

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Objective:To establish the experimental autoimmune encephalomyelitis(EAE) model on Guinea Pig with pan-spinal cord homogenate(coarse myelin basic protein, cMBP).

目的：用全脊髓匀浆（粗制髓鞘碱性蛋白）建立豚鼠实验性自身免疫性脑脊髓炎（EAE）模型。

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