The effects of concentration of NaOH and SDS temperature and duration of incubation on the isolation and purification of large plasmid DNA of Shigella were studied.
本实验采用碱变性的方法制备痢疾杆菌大质粒。在制备中观察了碱、温度、时间及SDS几个因素对质粒纯度的影响。
The effects of concentration of NaOH and SDS temperature and duration of incubation on the isolation and purification of large plasmid DNA of Shigella were studied.
本实验采用碱变性的方法制备痢疾杆菌大质粒。在制备中观察了碱、温度、时间及SDS几个因素对质粒纯度的影响。
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