METHODS Immunofluorescence histochemical double staining technique was used and the staining results were observed with confocal laser scanning microscope.
方法免疫荧光组织化学双重染色，染色结果在激光共聚焦扫描显微镜下观察。
Methods: The double labeling immunocytochemical staining technique was used.
方法：免疫细胞化学双重标记技术。
Methods Immunohistochemical staining combined with the micro-image analysis and immunofluorescence histochemical double-staining technique were used.
方法免疫组织化学染色结合显微图像定量分析和免疫荧光双重染色。
Methods Double label immunofluorescence histochemical staining and confocal laser_scanning microscope were used in the experiment.
方法应用免疫荧光组织化学双标记染色技术，在激光共聚焦扫描显微镜下观察染色结果。
METHODS: Immunofluorescence histochemical double-staining method was used.
方法：免疫荧光组织化学双重标记技术。
Methods: The staining method and washing step were reformed and tap-water was replaced by double distilled water.
方法：实验从染色冲洗的环节进行改良，采用蒸馏水(纯净水)取代自来水。
Methods Double labeling immunocytochemical staining technique was used.
方法采用免疫细胞化学双重标记技术。
Methods: Laser confocal double immunofluorescence staining method was used to detect the expression of PLP in the mature type-1 astrocytes, type-2 astrocytes and oligodendrocytes.
方法：用激光共聚焦双重免疫荧光标记技术检测PLP在分化成熟的两型星形胶质细胞和少突胶质细胞中的表达情况。
Methods: Laser confocal double immunofluorescence staining method was used to detect the expression of PLP in the mature type-1 astrocytes, type-2 astrocytes and oligodendrocytes.
方法：用激光共聚焦双重免疫荧光标记技术检测PLP在分化成熟的两型星形胶质细胞和少突胶质细胞中的表达情况。
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