Serum-free DMEM medium with high glucose works for us.
我们使用的是不含血清的高糖DMEM培养基，效果很好。
The isolated cells were cultured in DMEM medium and PDL fibroblasts were purified in subculture.
在DMEM培养基中进行了原代及传代培养，对其中的成纤维细胞进行分离纯化。
The cells were planked with lower cell density, and further screened in DMEM medium containing G418 MFD;
采用较低的细胞密度铺板，加入含最小致死量G418的DMEM培养基进行再次筛选；
After 3 passages were obtained, and then digested by DMEM medium containing fetal calf serum, cell amount was counted and the proliferation curve of MSCs was depicted.
取第3代骨髓基质细胞，以含胎牛血清的DMEM培养液培养消化后，计数细胞量，描绘细胞增殖曲线。
Meanwhile, normal NIH3T3 cells were taken as controls, and cultured by G418 contain MFD and by DMEM medium without G418 respectively. Light microscope was used to observe G418R NIH3T3 cells.
同时选用正常的NIH3T3细胞为对照，使用含有最小致死量g418及未加g 418的DMEM培养基进行培养，采用光学显微镜对G418抗性NIH3T3细胞进行观察。
Meanwhile, normal NIH3T3 cells were taken as controls, and cultured by G418 contain MFD and by DMEM medium without G418 respectively. Light microscope was used to observe G418R NIH3T3 cells.
同时选用正常的NIH3T3细胞为对照，使用含有最小致死量g418及未加g 418的DMEM培养基进行培养，采用光学显微镜对G418抗性NIH3T3细胞进行观察。
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