Serum-free DMEM medium with high glucose works for us.
我们使用的是不含血清的高糖DMEM培养基，效果很好。
Objective To compare the culture effects of CHO-C28 cells by domestic and imported DMEM.
目的比较国产与进口DMEM培养基培养cho - C28细胞的效果。
Results: (1) Sub-confluent cells having starved in DMEM containing 0.4% FBS for 48h, 88.5% of the cells were at G0/G1 phase of the cell cycle.
研究结果表明：（1）培养至次融汇状态的乳鼠心脏成纤维细胞经低血清（0.4％FBS）饥饿培养48小时，G0/G1期细胞占88.5％；
Conclusion Domestic DMEM was safe and effective for cell culture and reached the level of imported DMEM, thus was suitable for large-scale production of HB vaccine.
结论国产培养基在细胞培养中安全、有效，达到进口培养基水平，可用于规模化生产。
After 3 passages were obtained, and then digested by DMEM medium containing fetal calf serum, cell amount was counted and the proliferation curve of MSCs was depicted.
取第3代骨髓基质细胞，以含胎牛血清的DMEM培养液培养消化后，计数细胞量，描绘细胞增殖曲线。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H -DMEM培养液培养。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H -DMEM培养液培养。
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