First, direct sequencing of the 16s rRNA genes in patient isolates can allow rapid disease diagnosis.
第一，直接对病人提取物的16srRNA基因测序可以实现疾病的快速诊断。
Fig 3 Atlas of concordant results between DHPLC and direct sequencing. The arrows indicated the mutant peaks.
图3DHPLC与直接测序结果相符的检测图谱，箭头提示突变峰。
METHODS ITS1 and ITS2 genes of Pogostemon Cablin from different localities were identified by PCR direct sequencing.
方法采用PCR直接测序技术对不同产地的广藿香its1和ITS2基因进行测序分析。
This paper reports a new method to identify the Chinese drug turtle shells using PCR product direct sequencing method.
用PCR产物直接测序法对中药材龟甲（板）进行鉴别。
The results show that molecular identification based on PCR and direct sequencing is desirable to identify thrips species.
结果表明，基于PCR及直接测序技术的分子鉴定可以达到准确鉴定蓟马物种之目的。
The results show that molecular identification based on PCR and direct sequencing is desirable for identifying thrips species.
结果表明，基于PCR及直接测序技术的分子鉴定可以达到准确鉴定蓟马物种之目的。
Now the most common method to detect EGFR gene mutations was direct sequencing, expensive and time consuming, which didn't fit clinical screening.
目前检测EGFR基因突变的方法主要是直接测序，费时，费用较高，不适合用于临床筛查。
Using PCR-SSCP and PCR product direct sequencing techniques, we analysed the ORF region of SOX4 gene encoding in 15 lung cancer tissues and 8 normal controls.
本文采用PCR-SSCP及PCR产物直接测序等技术，对15例肺癌患者的组织标本及8例正常对照个体血细胞SOX4基因编码区进行了突变分析。
Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.
分别设计MSX1、PAX9基因特异性引物，聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列，产物纯化后直接测序。
Methods Sequences of HLA-DPB1, DRB exon2 of 10 samples were analysed by direct sequencing of PCR products. The results of sequencing were compared with their corresponding sequence data.
方法利用聚合酶链反应（PCR）产物直接测序，对10例血液标本进行了HLA-DPB1、DRB外显子2的序列分析，并将测定结果与基因型核酸序列数据库进行比较。
Conclusions: The new high resolution melting curve analysis is more efficient, more convenient than direct sequencing. Moreover, it is a low-cost test, which is suitable for clinic test.
结论：新的高分辨率熔解曲线分析法在检测KRAS基因突变时比直接测序法具有耗时短，易操作的优点，并且检测成本低廉，适合在临床开展。
Conclusions: The new high resolution melting curve analysis is more efficient, more convenient than direct sequencing. Moreover, it is a low-cost test, which is suitable for clinic test.
结论：新的高分辨率熔解曲线分析法在检测KRAS基因突变时比直接测序法具有耗时短，易操作的优点，并且检测成本低廉，适合在临床开展。
应用推荐