Mutation analysis was detected by direct sequencing RT-PCR products.
直接测序法检测RT PCR产物的基因变异；
First, direct sequencing of the 16s rRNA genes in patient isolates can allow rapid disease diagnosis.
第一，直接对病人提取物的16srRNA基因测序可以实现疾病的快速诊断。
Fig 3 Atlas of concordant results between DHPLC and direct sequencing. The arrows indicated the mutant peaks.
图3DHPLC与直接测序结果相符的检测图谱，箭头提示突变峰。
METHODS ITS1 and ITS2 genes of Pogostemon Cablin from different localities were identified by PCR direct sequencing.
方法采用PCR直接测序技术对不同产地的广藿香its1和ITS2基因进行测序分析。
This paper reports a new method to identify the Chinese drug turtle shells using PCR product direct sequencing method.
用PCR产物直接测序法对中药材龟甲（板）进行鉴别。
The results show that molecular identification based on PCR and direct sequencing is desirable to identify thrips species.
结果表明，基于PCR及直接测序技术的分子鉴定可以达到准确鉴定蓟马物种之目的。
The results show that molecular identification based on PCR and direct sequencing is desirable for identifying thrips species.
结果表明，基于PCR及直接测序技术的分子鉴定可以达到准确鉴定蓟马物种之目的。
Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing.
采用单链聚合酶链反应(PCR)和测序法检测肿瘤组织中VHL基因的突变情况。
Now the most common method to detect EGFR gene mutations was direct sequencing, expensive and time consuming, which didn't fit clinical screening.
目前检测EGFR基因突变的方法主要是直接测序，费时，费用较高，不适合用于临床筛查。
Using PCR-SSCP and PCR product direct sequencing techniques, we analysed the ORF region of SOX4 gene encoding in 15 lung cancer tissues and 8 normal controls.
本文采用PCR-SSCP及PCR产物直接测序等技术，对15例肺癌患者的组织标本及8例正常对照个体血细胞SOX4基因编码区进行了突变分析。
Direct sequencing showed the absence of mutations capable of interfering with viral replication and gene expression in the major viral population of each case.
直接测序显示每例患者主要的病毒株基因变异缺失都能干扰病毒复制和基因表达。
Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.
分别设计MSX1、PAX9基因特异性引物，聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列，产物纯化后直接测序。
Methods Genomic DNA was extracted from frozen tissues of 10 ameloblastomas and one malignant ameloblastoma. AMBN gene alterations were detected by PCR-direct sequencing.
方法收集10例成釉细胞瘤和1例成釉细胞癌的新鲜组织标本及相应外周血或牙龈黏膜组织，直接测序法分析AMBN基因；
These methods are particularly suited to screening antibody libraries and in preferred embodiments make use of mass spectrometry techniques for direct or indirect sequencing.
这些方法特别适合于筛选抗体文库，在优选实施方案中，利用质谱技术进行直接或间接测序。
Methods Sequences of HLA-DPB1, DRB exon2 of 10 samples were analysed by direct sequencing of PCR products. The results of sequencing were compared with their corresponding sequence data.
方法利用聚合酶链反应（PCR）产物直接测序，对10例血液标本进行了HLA-DPB1、DRB外显子2的序列分析，并将测定结果与基因型核酸序列数据库进行比较。
Conclusions: The new high resolution melting curve analysis is more efficient, more convenient than direct sequencing. Moreover, it is a low-cost test, which is suitable for clinic test.
结论：新的高分辨率熔解曲线分析法在检测KRAS基因突变时比直接测序法具有耗时短，易操作的优点，并且检测成本低廉，适合在临床开展。
He concluded that next generation sequencing will impact our knowledge about tumor behavior and have direct translational potential.
他推断下一步基因测序将会对我们对肿瘤行为知识产生影响，有直接翻译能力。
Conclusion MTC can be diagnosed at gene level by direct gene sequencing analysis. It is possible to diagnose MTC before operation by means of molecular genetic analysis.
结论直接基因测序分析能在基因水平诊断MTC，分子遗传学分析使术前诊断该疾病成为可能。
CONCLUSION: We first amplified mtDNA in circulating plasma from breast cancer patients by direct PCR, checked mutation by DHPLC and confirmed results by sequencing.
结论：本实验直接用PCR扩增乳腺癌血浆核酸中线粒体基因，并用DHPLC初步筛选突变并测序证实。
The sequencing of concrete grouting blocks on dam has a direct effect on the grouting progress and the uniformity of construction strength.
大坝混凝土浇筑块的排序方法影响其浇筑施工进度和施工强度的均衡性。
In contrast, direct DNA sequencing requires only a single sample from the proband.
相比之下，直接测序只需要被检测者的血样。
Methods Polymerase chain reaction(PCR)was used for 12 JAK2V617F -negative PV patients to amply the region of JAK2 exon 12, direct gene sequencing was performed to detect mutations of JAK2 exon 12.
方法采用聚合酶链反应（PCR）扩增12例JAK2V617F点突变阴性PV患者的JAK2外显子12片段，经基因测序与野生型JAK2外显子12比对，了解是否存在JAK2外显子12突变。
New methods of sequencing degraded fragments of DNA allowed scientists to make the first direct comparisons between the modern human genome and that of our Neandertal ancestors.
对DNA降解片段进行测序的新方法使得科学家们能够第一次对现代人基因组与我们的尼安德特人祖先的基因组进行直接的比较。
New methods of sequencing degraded fragments of DNA allowed scientists to make the first direct comparisons between the modern human genome and that of our Neandertal ancestors.
对DNA降解片段进行测序的新方法使得科学家们能够第一次对现代人基因组与我们的尼安德特人祖先的基因组进行直接的比较。
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