Methods Sweat glands epithelial cells were collected by enzymatic digestion.
方法酶消化法培养人汗腺上皮细胞。
Conclusion: The improved methods of manual purification, cool digestion and dermal scratching may increase the human keratinocyte livability and growth speed.
结论：采用人工纯化法、冷消化法和真皮刮除法等改良方法可提高体外培养的人表皮角质细胞存活率及生长速度。
Methods: the sample, after the nitric acid and the perchloric acid digestion was induced in the flame atom absorption spectrophotometer with standard series comparison quota.
方法：样品用硝酸和高氯酸消化后，导入火焰原子吸收分光光度计中，与标准系列比较定量。
Methods Rat gastric parietal cells were isolated by pronase digestion and purified by discontinuous density-gradient centrifugation.
方法采用链霉蛋白酶消化、间断密度梯度离心纯化大鼠胃黏膜壁细胞。
Material and Methods: From bovine eyes IPE cells were isolated with enzyme-assisted microdissection, corneal endothelium cells with enzyme digestion and then were cultured in vitro.
材料和方法：采用酶辅助的显微分离法分离新生牛眼ipe细胞，酶消化法分离角膜内皮细胞，进行体外细胞培养。
Methods: Graphite furnace atomic absorption spectrometry was used to determine cadmium content by microwave digestion sample.
方法：采用微波消解样品，石墨炉原子吸收法测定镉含量。
Methods IPE and RPE cells were isolated by enzymatic digestion method and enzyme-assisted microdissection method respectively.
方法采用酶消化法及酶辅助机械分离法分别分离IPE和RPE细胞。
Methods Reformed enzymatic digestion.
方法改良酶消化法。
Methods kidney cortex explanted into the serum-free culture medium gave rise to a primary culture of kidney epithelial cells. After confluence, cells were subcultured using Trypsin-EDTA digestion.
方法取肾皮质组织块，应用无血清培养液进行原代培养，融合后用胰蛋白酶-EDTA消化传代。
Results from two common methods for sample pretreatment: microwave digestion and traditional hotplate digestion were compared and discussed in the thesis.
在论文中以微波消化和传统加热板消化此两种常见的方式进行样品前处理，比较并讨论其分析结果。
METHODS: cranial neural tubes were dissected from 8.5 day mouse embryo using trypsin digestion and explanted in culture dishes. Cells dissociated from the neural tubes were cranial neural crest cells.
方法：采用胰酶消化法分离小鼠胚胎第8 . 5天的颅神经管，从小鼠颅神经管中游离出来的细胞即为颅神经嵴细胞。
For Navicula, a majority of diatoms could be extracted by enzymatic digestion method, while only a few diatoms could be detected by other three methods.
对舟形藻（海水），酶消化法的检出率高，其余三种方法几乎检测不到硅藻；
Methods Amnion cells from human were cultured with tissue piece method, passaged by trypsin digestion and identified with immunocytochemistry.
方法采用组织块培养法培养羊膜细胞，酶消化法对细胞进行传代，应用免疫细胞化学的方法对细胞进行鉴定。
Methods: the osteoblasts obtained from the calvaria of neonatal SD rats were cultured by method of enzyme digestion in vitro.
方法：采用酶消化法传代培养乳sd大鼠颅盖骨细胞并建立缺氧模型。
Methods Human fetal limbal epithelial tissue was cultured in vitro by enzymatic digestion culture method and the morphological characteristics of cultured cells were recorded.
方法应用消化培养法对人胎儿角膜缘上皮组织进行体外培养并观察记录培养细胞的生长特性；
Methods the enzyme directory digestion method was used to isolate Mesenchymal stem cells from Human Placenta and flow cytometry and karyotyping were used to evaluated the character of stem cells.
方法采用酶直接消化法从胎盘组织中提取间充质干细胞，并利用流式细胞术及染色体核型分析技术鉴定其干细胞特性。
Methods: 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction(PCR) technique.
方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（PCR）技术。
Methods: 1. In vitro digestion: Phosphomolybdic acid colorimetry;
方法：1 。体外消化实验磷钼酸比色法；
Methods: After a pretreatment with nitric acid or perchloric acid wet digestion, we used atomic fluorescence spectrometer methodology to detect selenium in human serum and urine.
方法：采用硝酸、高氯酸对尿样及血清进行湿法消解，利用原子荧光光谱法测定其中的硒含量。
Methods: Microwave digestion and atomic absorption spectrophotometer common water Fe and Mn content.
方法：采用微波消解和原子吸收分光光度法对常见水进行铁、锰含量测定。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
The standard plant cabbage was digested by different mixed acid and determined quantitatively by ICP-AES in this paper, the suitable methods of digestion for different elements were confirmed.
用不同混酸对植物标样甘蓝进行消解并用ICP-AES法对其进行了定量分析，确定了各元素适合的样品处理方法。
METHODS: Retinal vascular preparations were performed by using trypsin digestion and pepsin-trypsin digestion technique respectively.
方法：分别用胰蛋白酶消化法及胃蛋白酶-胰蛋白酶联合消化法制备视网膜血管铺片，比较铺片成功率。
Methods Microwave digestion of samples with different digestion conditions and atomic absorption spectrometry were used to determine Pb, As, cr, Cd and Cu contents in 75 kinds of cosmetics.
方法微波消解75种化妆品，原子吸收分光光度法测定化妆品中重金属铅、铬、砷、镉和铜的含量，试验了不同消解条件对消解效果的影响。
Methods: Urinary iodine of the 426 early pregnant women was determined by chloric acid-digestion and arsenic-cerium catalysis.
方法采用酸消化-砷铈接触法测定426例早期妊娠妇女尿碘。
Methods The mutation had been screened from 116 unrelated patients with type 1 diabetes mellitus by ApaI digestion of product of polymerase chain reaction amplification.
方法对116例随机收集的1型糖尿病患者用聚合酶链反应-限制性内切酶消化作该点突变的筛选；
Studies on pharmacodynamics of Xiaoerkang Granule(XEKG) was carried out by using the methods of strengthening the spleen, promoting digestion, alleviating pain and tranquilizing the mind.
为考察小儿康冲剂的健脾开胃、消食导滞、安神定惊功效，用健脾、消食、止痛、安神等方法对小儿康冲剂进行了药理研究。
Results in the culture of BCECs in vitro, the methods of separating posterior elastic layer and endothelium of cornea under microscope, combined with delay digestion proved to succeed easily.
结果显微分离后弹力膜及内皮细胞层联合延迟消化培养法，体外培养牛角膜内皮细胞成功率高。
Results in the culture of BCECs in vitro, the methods of separating posterior elastic layer and endothelium of cornea under microscope, combined with delay digestion proved to succeed easily.
结果显微分离后弹力膜及内皮细胞层联合延迟消化培养法，体外培养牛角膜内皮细胞成功率高。
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