The authors considered:(1)To design primers attention must be paid to evade UGA and to take place of UGG.
实验中我们体会到：（1）在设计引物时，要注意避开UGA，可用UGG代替。
We compare the genome of BV E2490 in NCBI with other herpes virus to find the sequence with diversity, and try to design primers in the sequences.
根据NCBI上发表的BVE2490株的基因组序列，将其与其他疱疹病毒科病毒的基因组进行比对，找出其中有差异的序列，根据这些序列设计引物。
Our method extended the utility of universal primers and provided implications for primer design and PCR amplification of some other genes.
新方法扩展了“通用引物”的适用范围，并为引物设计和一些其它基因的PCR放大提供了思路。
The overlap primers design is the key factor for the successful accomplishment of SOE PCR.
重叠延伸PCR技术成功的关键是重叠互补引物的设计。
The primer design is a key step for RAMP and REMAP, in which anchored simple sequence repeat primers were used.
引物设计是RAMP和REMAP标记分析的关键，二者都使用锚定的微卫星序列引物。
The results showed that: the design of primers can be amplified three parasites, and with the high specificity of the probe.
结果表明：设计的引物能够扩增三种寄生虫，而且探针的特异性很高。
Next work is to design the differential primers and transvert it to SCAR.
进一步工作是设计特异性引物，将其转化为SCAR标记。
According to the purpose of gene transcription design of the two primers.
根据转录出的目的基因设计两条引物。
Design and search primers for multiple applications including PCR, DNA hybridization and sequencing.
设计和搜索多重引物，包括PCR引物、序列探针和测序引物。
Design and search primers for multiple applications including PCR, DNA hybridization and sequencing.
设计和搜索多重引物，包括PCR引物、序列探针和测序引物。
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