Objective To generate rescued viruses with deletion mutation of capsid protein from dengue virus type 2 isolated in China (DEN2-43).
目的制备登革2型病毒中国分离株(DEN2 - 43)的衣壳蛋白缺失突变病毒。
Conclusion: This new method of DNA sequence deletion mutation is simple and accurate. It could avoid new mutation in PCR process of traditional DNA manipulation method.
结论：该方法操作简单、精确，能够避免引入新突变，为DNA序列的缺失突变提供了一种新方法。
Deletion mutation state of the two groups of mitochondria DNA were detected by PCR technology and photodensity scan.
采用聚合酶链反应（PCR）技术和光密度扫描检测两组线粒体DNA的缺失突变情况。
Deletion mutation state of the two groups of mitochondria DNA were detected by PCR technology and photodensity scan.
采用聚合酶链反应（PCR）技术和光密度扫描检测两组线粒体DNA的缺失突变情况。
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