It was beneficial to add acetosyringone into cocultivation medium for improving transformation frequency.
共培养培养基中添加乙酰丁香酮可提高转化频率。
FACS analysis demonstrated that about 25% of murine 5 FU prestimulated bone marrow cells could be infected after cocultivation with producing cells in vitro .
经包装获得较高滴度的病毒上清，以共培养的方式感染5-氟尿嘧啶预刺激的小鼠骨髓细胞。
FACS analysis demonstrated that about 25% of murine 5 FU prestimulated bone marrow cells could be infected after cocultivation with producing cells in vitro .
经包装获得较高滴度的病毒上清，以共培养的方式感染5-氟尿嘧啶预刺激的小鼠骨髓细胞。
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