Besides the low efficiency of cloning - just 1.7 per cent of embryos came to term - another challenge to creating transgenic dogs is controlling where in the nuclear DNA a foreign gene lands.
除了低克隆率-只有1.7%的胚胎能存活到最后阶段-创造转基因狗还有一个挑战即如何控制外部基因在核d NA中的植入位置。
The successful cloning of monkey HCBP6 gene paves a way for the study of the relationship of structure and function of HCBP6 genes from different species.
猴hcbp6同源基因的克隆化为研究不同种属来源的HCBP6基因的结构域功能、表达与调控等研究奠定了基础。
The cloning of human angiopoietin 1 gene and construction of it's expression vectors lay a good foundation of further study on the function, biological activity and application.
人血管生成素-1基因的克隆及表达载体的构建，为进一步研究其功能、活性和应用奠定了基础。
All in all, it is responsible to apply this kind of positional cloning strategy for finding the disease gene in this big family.
总之，本研究所采用的定位方法对于这个家系的致病基因的准确定位是合理的。
In recent years, some studies about gene cloning of this enzyme have got much achievement.
近几年猪肝酯酶的基因克隆研究也取得了较大进展。
Objective To construct cloning vector of HP450 gene.
目的构建HP450基因的克隆载体。
Objective Cloning of 14-3-3 antigen gene of Schistosoma japonicum in order to its study for immunological diagnosis and prevention.
目的克隆日本血吸虫14-3-3抗原的编码基因，以研究其用于血吸虫病免疫诊断和免疫预防效果。
This shows that a xylose isomerase gene was missing in the yeast, and cloning of this gene from other organisms might make the yeast ferment xylose directly.
这表明，它缺少异构酶基因，如果将其它微生物的这种基因克隆到酵母中去，它就能直接发酵木糖。
Some ways of cloning plant gene promoters were introduced, meanwhile the limitation of these ways was introduced and their developing prospect was also discussed in this paper.
本文着重介绍了几种植物基因启动子的克隆方法，分析了它们的优缺点，并展望了今后的研究前景。
The results show that it is a convenient technique for cloning new gene by searching EST database with homologous gene of model species such as fruit fly.
说明根据物种间同源基因序列，进行跨物种EST数据库的同源检索筛选、拼接，是基因克隆的一条有效途径。
This review introduced the structure and function of melatonin receptor 1b, the cloning and structure, developmental expression, mapping and polymorphism of melatonin receptor 1b gene.
作者介绍了褪黑激素受体1b的结构和功能，褪黑激素受体1b基因的克隆及基因结构、发育性表达与作用、定位与多态性，并讨论了该基因与繁殖季节性的关系。
Objective Molecular cloning and sequencing of the human nerve growth factor gene.
目的克隆人神经生长因子基因编码区。
Cloning of plant resistance gene is greatly helpful to crop resistance breeding and the insight of resistance mechanism.
植物抗病基因克隆研究对于抗病育种和抗病机制的理解具有重要意义。
Objective: Cloning of anti CD34 single chain antibody gene.
目的：获得抗人CD34单链抗体基因。
To study the biosynthetics genes of apramycin in Streptomyces tenebrarius, the apramycin resistant gene was isolated by gunshot cloning firstly.
为了研究黑暗链霉菌安普霉素生物合成基因，首先通过鸟枪克隆的方法克隆其安普霉素抗性基因。
The gene structure of antibacterial peptides and the research of cloning and expression of the genes are .
本文主要综述了抗菌肽基因的克隆与表达的研究进展并展望了抗菌肽在畜牧业生产中的应用前景。
These will provide abundant and reliable molecular markers for gene location and molecular cloning in the coming genetic engineering breeding of the strains.
并将为下一步的基因工程育种工作中的基因定位和分子克隆提供丰富可靠的分子标记。
This paper is an introduction of the higher plant NADP-ME in the aspect of cloning, function and regulation of gene expression.
文章就高等植物NADP - ME的克隆、功能和基因表达调控方面作一介绍。
The technology of cloning and gene manipulation makes the construction of idealized offspring possible, but do we have the maturity for it?
复制技术和基因改造的科技让我们有可能创造出理想的子孙，但我们技术成熟了吗？
It has many advantages, such as low investment, high pertinence, and it is fast, easy to operation, it will be the main strategy of functional genes cloning in plant gene engineering.
它具有投入低、速度快、技术要求低和针对性强等优点。因此，电子克隆技术必将成为植物基因工程中获得新基因的重要手段。
Cloning and identification a novel LMW-GS gene and studying its function are of great significance.
克隆和鉴定新的LMW- GS基因并研究其功能有着重要意义。
In this paper, the lysozyme on its structure, separation and purity, mechanism, gene cloning were reviewed. Medicinal application and developing foreground of lysozyme was also discussed.
本文综述了溶菌酶的结构特点、作用机制、分离纯化、基因克隆等方面的研究现状，并对其医药应用和发展前景做了展望。
The invention further discloses a method for cloning the gene sequence of trehalose-6-phosphate synthetase derived from selaginella pulvinata.
本发明还公开了一种上述来源于垫状卷柏的海藻糖-6-磷酸合成酶基因序列的克隆方法。
Conclusions: Bioinformatics analysis provided evidences for PPIL6 gene cloning and further study of the functions of PPIL6 gene.
结论：生物信息学分析为进一步进行PPIL6基因的克隆及功能研究奠定了基础。
Compared with Metarhizium anisopliae and Beauveria bassiana, the study on Verticillium lecanii is scarce and there have been few reports on cloning of infection related gene in this fungus.
相对于金龟子绿僵菌和球孢白僵菌而言，蜡蚧轮枝菌的研究十分薄弱，克隆该菌致病相关基因的报道至今不多。
It is a basic work for the application of marker-assisted selection and cloning of anti-disease gene in Japanese flounder selective breeding program.
这些抗病性候选标记的获得为实现牙鲆分子标记辅助育种和抗病基因克隆奠定了一定基础。
The condition of HSF1 gene silence, the survival rate of cells which was in RNAi was much lower than control group by cloning.
在HSF1基因沉默条件下，克隆法观察到转染后的细胞其细胞存活率比未转染的空白对照组明显降低。
Result shows that cloning purpose fragment has the function of promoter, and it can regulate lower reaches gene expression intensely in drought, high-salt and cold.
结果表明克隆到的目的片段具有启动子功能，并且在干旱、高盐、低温诱导胁迫下更能强烈地驱动下游基因的表达。
Objectives to construct the cloning vector of glycoprotein E1 gene of rubella virus, to study the mutation of glycoprotein E1 gene, and to analyze the sequence of E1 gene by the phylogenetic tree.
目的建立风疹病毒包膜糖蛋白e1的克隆载体；研究e1基因变异情况，并对其序列进行系统发生树分析。
Objectives to construct the cloning vector of glycoprotein E1 gene of rubella virus, to study the mutation of glycoprotein E1 gene, and to analyze the sequence of E1 gene by the phylogenetic tree.
目的建立风疹病毒包膜糖蛋白e1的克隆载体；研究e1基因变异情况，并对其序列进行系统发生树分析。
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