Donor cell selection is critical for the success of cloning animal, and it is also an important factor affecting the cloning efficiency.
但动物克隆效率仍然很低，供体细胞的选择是动物克隆中的重要步骤。
However, unfortunately, poor cloning efficiency and unclear understanding of key mechanisms underlying nuclear reprogramming seriously limit wider USES of animal cloning.
但是，体细胞克隆的效率仍不理想，关键机制还不清楚，严重制约了该技术的推广。
The changes of morphology, death rate, the inhibition rate of cell growth, cloning efficiency were observed. DNA content of MGC-803 were measured with the laser scanning confocal microtechnic.
计算各组细胞死亡率、细胞生长抑制率、克隆形成率并观察形态学变化，应用凝胶电泳、激光扫描共聚焦显微镜观察分析小叶黑柴胡作用MGC- 803细胞后的细胞DNA含量的改变。
Besides the low efficiency of cloning - just 1.7 per cent of embryos came to term - another challenge to creating transgenic dogs is controlling where in the nuclear DNA a foreign gene lands.
除了低克隆率-只有1.7%的胚胎能存活到最后阶段-创造转基因狗还有一个挑战即如何控制外部基因在核d NA中的植入位置。
But, the efficency of cloning of PCR product is very poor because of the insufficiently cutting of two points of PCR fragment due to the low cutting efficiency of PCR product.
但是，由于PCR产物的酶切效率很低，致使片段两端不能够被完全切开，最终使得PCR产物的克隆效率较低。
Finally, we give the method of integrating in-place cloning and out-place cloning in order to further improve the efficiency of generalized multi-scenario analysis simulation.
最后提出综合集成原地克隆和异地克隆的方法，更加高效地支持广义多方案分析仿真。
Specific primer PCR was a gene cloning technique with high efficiency, stability and accuracy.
特异引物PCR方法是一种高效、稳定、可靠的基因克隆手段。
Specific primer PCR was a gene cloning technique with high efficiency, stability and accuracy.
特异引物PCR方法是一种高效、稳定、可靠的基因克隆手段。
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