The clone and expression of cellulase gene of trichoderma spp.
木霉纤维素酶基因的克隆与表达研究进展。
Conclusion Successful clone of an integrity SARS-CoV nucleocapsid gene and its effective expression in saccharomyces cerevisiae can be beneficial to the research on SARS vaccine.
结论：成功克隆SARS冠状病毒核衣壳蛋白基因全长，并在酿酒酵母中诱导表达成功，有助于抗sars分子疫苗的研究。
Aim to analyze THANK gene expression in peripheral blood mononuclear cells (PBMC) stimulated with different stimulators and to clone whole-length human THANK gene.
目的分析不同刺激下外周血单个核细胞(PBMC)中THANK基因的表达，并克隆全长的人THANK基因。
One of the keys to understand the molecular mechanism of gene expression and the pathway of signal transduction is to clone and identify the transcription factors.
要阐明各种信号传递途径与基因表达调控的机理，克隆和鉴定转录因子是关键。
Aim to clone human CD81 gene from peripheral blood lymphocytes, and construct its eukaryotic expression vector, and then express it in COS-7 cells.
目的从人外周血淋巴细胞中克隆出cd 81基因，构建真核表达质粒，并在COS - 7细胞中进行表达。
AIM: to clone rat neurotrophin-4 (NT-4) total gene and construct expression plasmid for prokaryotic cells.
目的：克隆大鼠神经营养因子4全长基因，构建真核细胞表达质粒。
Objective:To clone full-length FKN gene of human, chimpanzee and leaf monkey and compare their difference in genome and protein expression level.
目的克隆人、黑猩猩和叶猴FKN全基因及体外表达，比较研究FKN在进化过程中基因组水平和蛋白表达水平的差异。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective To identify and clone the gene encoding human ubiquitin binding enzyme and study its expression spectrum.
目的分离、克隆人泛素结合酶基因，并初步研究其组织表达谱。
Objective: to clone the human and murine origin RANTES genes and construct the adenoviral expression vectors.
目的：克隆人和小鼠的活化t细胞表达与分泌调节基因(RANTES基因)并分别构建腺病毒表达载体。
AIM To clone human heat shock protein 72 (HSP72) gene, do prokaryotic expression and purify HSP72 protein for further study.
目的克隆人热休克蛋白72 (HSP72)基因，原核表达并纯化蛋白产物，以探讨其生物学功能。
To clone mouse interleukin 4 (mIL-4) truncated gene, construct its eukaryotic expression plasmid pFB-mIL4 and express the truncated protein (murine IL-4 receptor antagonist).
构建小鼠il -4截短型基因真核表达质粒，表达小鼠il - 4受体拮抗体蛋白。
Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid.
目的克隆人血管紧张素转换酶2基因（ACE2），并构建其真核表达载体。
In modern research of molecular biology, we usually need to analyse or clone these flanking sequence in given sites, so as to study gene expression and control.
在现代分子生物学技术研究中，常常需要对已知位点的侧翼序列进行分析或克隆，以研究基因的遗传表达调控。
Objective To clone BMP7 gene in vitro, and determine the expression of BMP7 gene in articular chondrocytes of rabbits cultivated in vitro.
目的：克隆骨形态发生蛋白bmp7基因并检测其在体外培养的兔关节软骨细胞中的表达。
ObjectiveTo clone the gene of mortality factor related gene 15(MRG15) and to investigate its expression in lens epithelium cell(LEC) of normal and age related cataractous eyes.
目的克隆死亡因子相关基因15（MRG15）并研究其在正常晶状体和年龄相关性白内障晶状体上皮细胞中的表达，比较二者的差异。
AIM: to clone human MAGE 3 gene, to induce its prokaryotic expression and to purify the protein.
目的：克隆人MAGE3基因并进行原核表达和分离纯化。
Objective to clone the gene activating transcription factor 5 (ATF5), construct the expression plasmid and detect the localization of ATF5 in cultivated cells.
目的克隆激活转录因子(atf)5，构建其真核表达载体，观察其在细胞中的表达定位。
To clone gene of the TRAIL and construct its prokaryotic expression vector.
目的克隆人TRAIL基因，构建其原核表达载体。
Objective To clone human epidermal growth factor receptor (HER2) gene and establish a MCF-7 cell line with stable co-expression of HER2 and estrogen receptor (ER).
目的克隆人表皮生长因子受体（HER2）基因，建立共表达HER2基因和雌激素受体（ER）基因的MCF-7细胞模型。
Conclusions a clone of murine GAD65 is successfully constructed. It is a basis step for further construction of expression plasmid that expresses GAD65 and for immunologic intervention of T1DM.
结论成功的克隆了小鼠gad65基因，为构建GAD65基因疫苗以及糖尿病的免疫干预治疗打下基础。
Gain many clone of gene fragment on differential expression, choice 3 of them for sequencing, and blast the sequence in Genebank.
获得了很多差异片段的克隆，并从其中选择3个进行测序。
Gain many clone of gene fragment on differential expression, choice 3 of them for sequencing, and blast the sequence in Genebank.
获得了很多差异片段的克隆，并从其中选择3个进行测序。
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