The patch clamp technique has become the main method for cell electrophysiology research.
膜片钳技术已成为细胞电生理学研究的重要手段。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
METHODS The patch clamp whole cell recording technique was used.
方法采用膜片钳全细胞式记录技术。
METHODS Patch clamp technique with whole cell configuration.
方法膜片钳制技术全细胞记录模式。
The patch clamp technique has become the main method for the molecule and cell research.
膜片钳技术已成为分子和细胞学研究的重要手段。
Methods Cell-attached mode of patch clamp technique were employed to record the single channel current of NMDA receptor with the primary-cultured hippocampal neurons from rats as samples.
以原代培养的大鼠海马神经元为标本，运用膜片钳技术的细胞吸附式方法记录NMDA通道的单通道电流。
Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
Methods: Patch clamp whole cell recording technique was used.
方法：膜片钳全细胞电流记录方法。
Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.
方法：运用全细胞膜片钳技术记录持续性钠电流，并观察其在缺氧-复氧模型下的变化。
Methods: By using patch clamp technique, measure voltage gated calcium currents in undifferentiated SH SY5Y cell line.
方法：通过膜片钳手段，测量未分化的SHSY5Y细胞钙离子通道电流；
METHODS Voltage gated potassium outward current was recorded using whole cell patch clamp techniques in the cultured rat hippocampal neurons.
方法在培养的大鼠海马神经元上用膜片钳全细胞记录钾通道电流。
Methods Effects of quinidine and verapamil on the alteration of action potential duration induced by aconitine were studied by use of the whole cell patch clamp techniques in rat ventricular myocytes.
方法应用全细胞膜片钳技术记录乌头碱对大鼠心肌细胞动作电位时程的作用，分别给予奎尼丁、维拉·帕米处理比较二者对动作电位时程的影响。
METHOD The whole cell patch clamp techniques were used.
方法采用全细胞膜片钳技术。
Method The whole cell patch clamp method was used.
方法应用全细胞膜片钳制技术。
Methods the chloride channel currents were recorded in cultured A549 cells by using the whole-cell mode of patch clamp techniques.
方法在离体培养的A549细胞上，用膜片钳全细胞模式记录氯通道电流。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
方法：采用全细胞膜片钳技术，记录钠通道电流。
METHODS The whole cell patch clamp techniques were used.
方法全细胞膜片钳技术。
Methods: The patch clamp was used to record the changes in average whole cell current and the conductance of GABA-A receptor on cultured hippocampal neurons in different developing stages.
方法用膜片钳记录仪在体外培养的不同时期，记录大鼠海马神经元GABA-A受体离子通道全细胞通道电流、电导的变化。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
METHOD Whole cell patch clamp techniques.
方法全细胞膜片箝技术。
Results: (1) Whole-cell patch-clamp recording technique was established, and highly stable whole cell ion channels' current curves of normal ventricular myocytes were ploted.
结果：(1)建立了膜片钳全细胞记录技术平台，获得了高稳定性的正常心室肌细胞全细胞离子通道电流图。
Mechanical dissociation was used to obtain single primary spermatocytes from guinea pigs and iron channel currents in the primary spermatocytes were recorded by whole cell patch-clamp technique.
笔者应用机械分离获得豚鼠单个初级精母细胞，采用全细胞式膜片钳技术记录该细胞离子通道电流。
METHOD Whole-cell patch clamp technique was used.
方法采用全细胞膜片钳技术。
METHODS Using cell-attached patch clamp technique.
方法采用细胞贴附式膜片钳技术。
Whole-cell patch clamp technique was used to study the electrophysiological characteristics of nociceptive trigeminal ganglion (TG) neurons labeled by nuclear yellow (NY).
实验利用全细胞膜片钳技术研究了荧光染料核黄（NY）标记的支配牙髓的痛感觉神经元的电生理特征。
Methods Whole-cell patch clamp technique was used.
方法采用全细胞膜片钳制技术。
Methods: The isolated neurons of dorsal root ganglion (DRG) were studied using the whole cell patch clamp technique in current clamp mode.
方法：应用全细胞膜片箝电流箝制技术，对急性分离的大鼠DRG细胞进行电流箝制。
Methods The whole-cell patch clamp with current clamp was used.
方法采用全细胞膜片钳技术，进行电流钳制。
METHODS Whole cell recording by patch clamp technique.
方法膜片钳全细胞记录。
METHODS: The date recording from cell - attached patches of patch - clamp technique.
方法：采用细胞贴附式的膜片钳技术。
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