There was no cell clone observed in soft agar.
软琼脂培养无克隆形成。
Somatic cell clone has been applied successfully in sheep, goat, bovine, swine and so on.
体细胞克隆在绵羊、山羊、牛、猪等家畜中获得了成功，但目前的克隆效率非常低。
There was no obvious difference in the cell clone efficiency between human fetal eccrine sweat gland cells (7.3%) and human fetal keratinocytes (7.7 %, P>0.05).
人外泌汗腺细胞的克隆形成率为7.3%，与人胎儿角质形成细胞（7.7%）比较差异无统计学意义（P>0·05）。
The aim of this study was to characterize the cardiac differentiation potential of a murine iPS cell clone in comparison to a well-established murine es cell line.
本研究的目的是比较小鼠多功能干细胞克隆与胚胎干细胞系的心脏分化潜能。
It is showed that the cell clone-forming rat was 31. 2%. The immortalized human fetal hepatocytes had a normal karyotype and were not able to grow in soft AGAR culture.
结果显示永生化胚胎肝细胞克隆形成率为31.2%，染色体核型分析表明细胞核型无明显异常，软琼脂集落形成试验表明细胞在软琼脂中不能生长。
Conditioned medium (CM) stemming from cell suspension culture could remarkably promote the formation of single cell clone from culture cells of Panax ginseng below a critical cell plating density.
来自细胞悬浮培养物的条件培养基能显著地促进人参培养细胞的单细胞在较低细胞植板密度培养时的克隆形成。
The Workload Manager (WLM) in WebSphere does load balancing and can direct traffic to any available clone in a cluster or cell defined for the application.
WebSphere中的工作负载管理器（Workload Manager，WLM）执行负载平衡，并且可以将流量定向到集群或单元中为应用程序定义的任何可用副本。
Therefore, each initially stimulated B cell produces a clone of plasma cells, all producing the same specific antibody.
因此，最初每个被激活的B细胞产生1对浆细胞，二者都产生相同的特异性抗体。
NBAC members also indicated that they would appeal to privately funded researchers and clinics not to try to clone humans by body cell nuclear transfer.
NBAC的成员明确表示，他们呼吁由私人提供资金的研究人员和机构不要试图通过人体细胞核转移去克隆人。
Clone made the main steps of extraction of a cell from human or animals, through a complex process of slowly training and maternal identical organisms.
克隆制作的主要步骤是从人体或动物身上提取一个细胞，经过复杂的过程慢慢培养和母体一模一样的生物体。
But to make a true clone a cell needs to be not just pluripotent, but totipotent and thus able to turn into an entire animal.
但是为制作真正的克隆，一个细胞不仅要是多功能的，还必须是全能的，进而能转化成完整的动物。
AIM: to clone and analyze the mouse LASS1 gene promoter, so as to lay a foundation for the further research of the transcriptional regulation of LAG1 gene in cell senescence.
目的：克隆并应用计算机分析小鼠lass1基因启动子，为进一步研究在细胞衰老过程中LAG1基因的转录调控奠定基础。
A clone system designated SAC was established from a SRS-82 mouse ascitic turnout cell line by using limited dilution method.
应用有限稀释法，由小鼠srs- 82腹水瘤细胞系分离得到SAC克隆株系列。
Methods: Clone assay, MTT colorimetric assay and survival cell count assay were adopted.
方法：采用克隆形成法、MTT 比色法及活细胞计数法。
Methods Improved PCR-based subtractive hybridization was used to clone the genes from apoptotic prostatic carcinoma cell line DU-145 cells induced by ATRA.
方法：应用全反式维甲酸诱导前列腺癌DU 145细胞凋亡，建立细胞凋亡模型，采用基于PCR的改良消减杂交技术克隆与凋亡相关的基因。
Objective To clone the apoptosis-related genes induced by all trans-retinoic acid(ATRA) from prostatic carcinoma cell line DU-145, and investigate the molecular mechanism of the apoptotic process.
目的：克隆全反式维甲酸诱导前列腺癌细胞系DU 145细胞产生凋亡的相关基因。
METHOD The fluorescence simple clone stream mode cell meter was used.
方法采用荧光标记单克隆抗体流式细胞仪测定。
These results suggested that tumor cell are consist of sub clone of difference in biological characteristics.
这一结果提示：肿瘤细胞是由生物学特性不同的细胞亚群组成。
The influence of each vector on the cell proliferation, clone-formation and alteration of cell cycle of bladder cancer cell line EJ were observed in vitro.
分别转染膀胱癌细胞株EJ ，观察不同载体对膀胱癌细胞体外增殖、克隆形成和细胞周期时相的影响。
Objective To clone human epidermal growth factor receptor (HER2) gene and establish a MCF-7 cell line with stable co-expression of HER2 and estrogen receptor (ER).
目的克隆人表皮生长因子受体（HER2）基因，建立共表达HER2基因和雌激素受体（ER）基因的MCF-7细胞模型。
ObjectiveTo clone the gene of mortality factor related gene 15(MRG15) and to investigate its expression in lens epithelium cell(LEC) of normal and age related cataractous eyes.
目的克隆死亡因子相关基因15（MRG15）并研究其在正常晶状体和年龄相关性白内障晶状体上皮细胞中的表达，比较二者的差异。
NBAC members also indicated that they will appeal to privately funded researchers and clinics not to try to clone humans by body cell nuclear transfer .
NBAC的成员还表示，他们将呼吁由私人提供资金的研究人员和机构不要试图通过人体细胞核转移去克隆人。
Chronic myelocytic leukemia (CML) is the malignant clone disease which occurs in a haematopoietic stem cell.
慢性粒细胞白血病CML是造血干细胞的恶性克隆增生性疾病。
SH-SY5Y cells are an adrenergic clone of the human neuroblastoma cell line SK-N-SH and a very useful model for the study of neuronal cell terminal differentiation.
SY5Y细胞是人神经母细胞瘤细胞系SK-N-SH中的一个克隆，它是研究神经细胞终末分化的一个极有用的模型。
Method: the cell proliferation curve and the clone forming approaches were adopted.
方法：采用生长曲线和集落形成实验。
Positive clone were subcloned to get monoclone cell lines by limit dilution, repeat 3 times, then we got two positive monoelone cell lines named:2E8,3C6.
采用有限稀释法对阳生孔进行亚克隆，重复3次，得到两株单克隆细胞株：2E8、3C6。
Clone formation ability was determined by clony forming assay. Cell cycle and apoptosis were tested by flow cytometry.
通过流式细胞术检测细胞的细胞周期分布和凋亡水平；
Clone formation ability was determined by clony forming assay. Cell cycle and apoptosis were tested by flow cytometry.
通过流式细胞术检测细胞的细胞周期分布和凋亡水平；
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