• We have isolated a novel immunoglobulin type cell adhesion molecule, DSCAML1, from a fetal brain cDNA library.

    在胎脑文库中获得的DSCAML1属于免疫球蛋白超家族类型的细胞粘附分子。

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  • The plasmids in cDNA library and in gene pools were extracted and NIH/3T3 cells were transfected respectively.

    将文库菌落印迹至尼龙膜,分区培养提取质粒DNA ,建立基因池,并分别转染NIH/3T3细胞。

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  • The human novel gene of ZNF322 is cloned from human fetal cDNA library using the primers based on the ZNF322 sequence analyzed with computer.

    根据计算机克隆的ZNF322基因序列设计引物,从人类胚胎心脏文库中克隆了ZNF322基因。

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  • Some defense-relate genes were identified with cDNA library, they are possible related to resistances against coldness, drought etc in jujube.

    测序得到了许多与抗逆性有关的蛋白,这可能与枣树本身抗寒,抗旱等抗逆性强有关。

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  • RESULTS: human NS5ABP37 was screened and cloned from human liver cDNA library by yeast-two hybrid system 3. The murine NS5ABP37 was deduced by bioinformatics methods.

    结果:通过酵母双杂交技术获得了人ns5abp37的编码基因。利用生物信息学技术确定了小鼠ns5abp37的基因序列。

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  • Results A protocol for RACE cDNA library construction from bone and joint was established and two RACE cDNA libraries from human fetal bone and joint were successfully constructed.

    结果建立了从骨骼和关节构建RACE c DNA文库的方法,并用该方法成功地构建了人胎儿骨骼和关节RACE c DNA文库。

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  • Objective To construct subtracted cDNA library in hypothalamus of the seasickness adaptive rats for providing theoretical basis for effective adaptive training against seasickness.

    目的分离晕船适应大鼠下丘脑差异表达基因片段,以探讨晕船适应机理。

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  • Currently, several approaches have been developed for gene enrichment on a genome-wide scale, such as cDNA library, methylation filtration library, high Cot library and transposon tagging.

    目前,在基因组范围内富集基因的方法有cD NA文库、甲基化过滤文库、高Cot值文库、转座子标签富集法等。

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  • A library containing the genes that could be expressed as proteins would be referred to as a cDNA expression library, which could be screened for a protein that binds a particular antibody.

    一个包含可表达为蛋白质的基因的文库称为cDNA表达文库,它可以用来筛选结合特定抗体的蛋白。

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  • Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.

    目的:消减文库构建过程中,用P CR技术快速筛选重组阳性克隆。

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  • Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.

    筛选重组阳性克隆可直接用细菌悬液作PCR模板,在消减文库构建时,能大大提高工作效率。

    youdao

  • Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.

    筛选重组阳性克隆可直接用细菌悬液作PCR模板,在消减文库构建时,能大大提高工作效率。

    youdao

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