A library containing the genes that could be expressed as proteins would be referred to as a cDNA expression library, which could be screened for a protein that binds a particular antibody.
一个包含可表达为蛋白质的基因的文库称为cDNA表达文库,它可以用来筛选结合特定抗体的蛋白。
This genetic material can be in the form of a gene, a representative of a gene or cDNA, RNA or even a small fragment of a gene.
这种基因材料可以是基因,基因替代物或cDNA、RNA甚至小的基因片段。
RACE could be used to clone the full-length cDNA effectively on the basis of obtaining partial sequence.
在获得一定长度片段的序列基础之上利用RACE法可以有效地进行全长的克隆。
Aim: Cloning and sequencing of hOP-1 cDNA encoding mature protein.
目的:克隆成骨蛋白-1(OP-1)成熟蛋白编码区基因。
Based on sequence homology, it was suggested that the obtained cDNA was a SAM gene in tea plant.
证实所获得的基因序列为茶树SAM合成酶基因。
Objective: To clone cDNA of enamel matrix serine proteinase (EMSP1) encoding mature protein from mouse dental germs.
目的:克隆小鼠牙胚组织中釉基质丝氨酸蛋白酶(EMSP1)成熟肽编码区基因。
Objective: to study the effect of dioscin to breast cancer cytogene expression by cDNA microarray technique, and to explore target genes of drug action initially.
目的:通过基因芯片技术研究薯蓣皂苷对乳腺癌细胞基因表达的影响,初步探索药物作用的靶基因。
CONCLUSION: cDNA microarray can be used to screen diversified gene expression related to injury under ischemia and anoxia, which brings some new clues for studying the mechanism of IECs injury.
结论:应用基因芯片技术筛选了与IEC缺血缺氧损伤密切相关的差异表达基因,为阐明这方面的机制提供了新的线索。
A watermelon mosaic virus 2 (WMV-2) isolate from diseased watermelon in Shanxi Province was used for cDNA synthesis of WMV-2 CP gene by RT-PCR.
本研究以从山西西瓜上分离纯化得到的西瓜花叶病毒2号(WMV-2)分离物为材料,用RT-PCR方法得到其外壳蛋白(CP)。
Conclusion cDNA microarray technology can be successfully applied to identify differentially expressed genes with small amount of specimen, high quality, high speed, and high sensitivity.
结论用基因芯片筛选正常脑组织与人脑胶质瘤差异表达的基因,具有样品用量少,高质量,高速度,高敏感等特性。
Objective To explore the tumor related molecular mechanism and functional message from the gigantic cDNA array gene data.
目的探讨从巨量的微阵列基因数据中挖掘肿瘤相关分子机理及功能信息;
Conclusion: cDNA microarray found the tumor specific gene expression spectrum in gliomas.
结论:基因芯片发现了胶质瘤的异常基因表达谱。
Comparing the gene cDNA and amino acid sequences with other lactase sequences from various sources, it showed a very low homology with most of other sequences.
将此基因与不同来源的乳糖酶基因序列进行比较发现,该基因与绝大多数乳糖酶基因同源性较低。
Objective To investigate the gene expression profiles in lung adenocarcinoma (LA), tumor adjacent tissue (TAT) and fetal lung tissue (FLT) by cDNA microarray technique.
目的利用基因芯片技术研究肺腺癌组织、癌旁组织及胚胎肺组织中的基因表达差异。
Objective To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.
目的利用基因表达谱芯片对NB 4细胞在硫化砷作用前后基因表达的差异性进行比较。
Objective: Cloning CD4 cDNA of Homo sapiens to study the mechanism of virus infecting the CD4 + cell for further research.
目的:克隆人的CD 4基因,为进一步研究病毒感染CD 4 +细胞的分子机制奠定基础。
Objective: To study the expressions of pro-onc and tumor suppressor genes in gastric cancer by cDNA expression array.
目的:从基因水平了解正常胃和胃癌组织原癌基因和抑癌基因表达的分子机制。
Aim: To clone MAR-binding protein cDNA fragment from Dunaliella salina.
目的:克隆杜氏盐藻核基质附着区(MAR)结合蛋白片段。
Conclusion: The cloned sequence is probably MAR binding protein cDNA fragment from Dunaliella salina.
结论:所克隆的序列可能为杜氏盐藻MAR结合蛋白片段。
Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the AGAR gel electrophoresis.
最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。
Objective: To construct and identify recombinant adeno-associated virus encoding rat tissue inhibitor of metalloproteinase-1(TIMP-1) full length cDNA.
目的:构建及鉴定载入金属蛋白酶组织抑制因子1(TIMP鄄1)的重组腺相关病毒载体。
Objectives To study the difference between gene expressions of high (H0-8910PM) and low (HO-8910) metastatic human ovarian carcinoma cell lines and screen novel associated genes by cDNA microarray.
目的用基因芯片技术研究高低转移人卵巢癌细胞系(HO 8910 PM和HO 8910)基因表达谱差异,筛选与转移相关的基因。
RESULTS: human NS5ABP37 was screened and cloned from human liver cDNA library by yeast-two hybrid system 3. The murine NS5ABP37 was deduced by bioinformatics methods.
结果:通过酵母双杂交技术获得了人ns5abp37的编码基因。利用生物信息学技术确定了小鼠ns5abp37的基因序列。
According to the sequence characterization of zinc finger protein and other transcription factors, 58 EST sequences were selected from EST database of a 10 K rice cDNA chips.
我们根据锌指蛋白等转录因子特征结构域的序列特点,从来自10K水稻芯片的EST数据库中筛选出编码58个EST序列。
Results A protocol for RACE cDNA library construction from bone and joint was established and two RACE cDNA libraries from human fetal bone and joint were successfully constructed.
结果建立了从骨骼和关节构建RACE c DNA文库的方法,并用该方法成功地构建了人胎儿骨骼和关节RACE c DNA文库。
Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
目的:消减文库构建过程中,用P CR技术快速筛选重组阳性克隆。
Objective To in ve stigate the difference of immuno-related gene expressions in T lymphocyte line (HUT78) incubated with melatonin by cDNA microarray of gene expression profile.
目的利用基因表达谱芯片研究T淋巴细胞株(HUT78)在褪黑素作用后免疫相关基因表达的差异性。
Differential DNA dot hybridization of subtractive cDNA clones.
差减阳性克隆的斑点杂交。
Aim To investigate the effect of fibroblast growth factor 2 (FGF2) cDNA mediated by recombinant adeno-associated viral vector2 (rAAV-2) on ischemic myocardium.
探讨重组2型腺相关病毒载体介导2型成纤维细胞生长因子基因诱导家兔缺血心肌血管生成的作用。
Aim To investigate the effect of fibroblast growth factor 2 (FGF2) cDNA mediated by recombinant adeno-associated viral vector2 (rAAV-2) on ischemic myocardium.
探讨重组2型腺相关病毒载体介导2型成纤维细胞生长因子基因诱导家兔缺血心肌血管生成的作用。
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