A library containing the genes that could be expressed as proteins would be referred to as a cDNA expression library, which could be screened for a protein that binds a particular antibody.
一个包含可表达为蛋白质的基因的文库称为cDNA表达文库,它可以用来筛选结合特定抗体的蛋白。
Differential DNA dot hybridization of subtractive cDNA clones.
差减阳性克隆的斑点杂交。
Aim: Cloning and sequencing of hOP-1 cDNA encoding mature protein.
目的:克隆成骨蛋白-1(OP-1)成熟蛋白编码区基因。
Aim: To clone MAR-binding protein cDNA fragment from Dunaliella salina.
目的:克隆杜氏盐藻核基质附着区(MAR)结合蛋白片段。
The lengths of the double stranded cDNA fragments were about 500 base pairs.
其双链链长,经凝胶电泳分析,约为500碱基对。
AIM: To clone and analyze the cDNA encoding immunodiagnostic antigen of cysticercosis.
目的:克隆与分析囊虫病诊断用抗原及其编码基因。
Conclusion: cDNA microarray found the tumor specific gene expression spectrum in gliomas.
结论:基因芯片发现了胶质瘤的异常基因表达谱。
Based on sequence homology, it was suggested that the obtained cDNA was a SAM gene in tea plant.
证实所获得的基因序列为茶树SAM合成酶基因。
Study of the changes of gene expression during the process of liver fibrosis by cDNA microarray;
应用微矩阵表达谱基因芯片筛选食管癌新的相关基因。
Conclusion: The cloned sequence is probably MAR binding protein cDNA fragment from Dunaliella salina.
结论:所克隆的序列可能为杜氏盐藻MAR结合蛋白片段。
RACE could be used to clone the full-length cDNA effectively on the basis of obtaining partial sequence.
在获得一定长度片段的序列基础之上利用RACE法可以有效地进行全长的克隆。
We have isolated a novel immunoglobulin type cell adhesion molecule, DSCAML1, from a fetal brain cDNA library.
在胎脑文库中获得的DSCAML1属于免疫球蛋白超家族类型的细胞粘附分子。
Objective: To clone cDNA of enamel matrix serine proteinase (EMSP1) encoding mature protein from mouse dental germs.
目的:克隆小鼠牙胚组织中釉基质丝氨酸蛋白酶(EMSP1)成熟肽编码区基因。
The differential gene expression induced by overexpressed CT120B was investigated using Atlas cDNA expression array.
表达阵列分析显示过表达CT120B诱导的基因表达谱变化;
Objective: To study the expressions of pro-onc and tumor suppressor genes in gastric cancer by cDNA expression array.
目的:从基因水平了解正常胃和胃癌组织原癌基因和抑癌基因表达的分子机制。
Objective: Cloning CD4 cDNA of Homo sapiens to study the mechanism of virus infecting the CD4 + cell for further research.
目的:克隆人的CD 4基因,为进一步研究病毒感染CD 4 +细胞的分子机制奠定基础。
Objective To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.
目的利用基因表达谱芯片对NB 4细胞在硫化砷作用前后基因表达的差异性进行比较。
RESULTS:The results revealed that the sequence of kidney ASBT cDNA gene was identical to that of human intestine ASBT gene.
结果:序列分析结果表明肾小管ASBT基因的序列与小肠ASBT序列完全一致。
Differences in gene expressions were compared by cDNA microarray in skeletal muscle of type 2 diabetic rats and normal rats.
用基因芯片技术比较2型糖尿病大鼠和正常大鼠骨骼肌基因表达谱的差异。
Objective: To investigate the gene expression profiles of rat liver tissue injured by rifampicin (RFP) using cDNA microarrays.
目的:利用基因芯片技术研究抗结核病药物利福平对大鼠肝脏毒性效应的分子机制。
Objective: To clone and sequence a cDNA encoding fibrinolysin metalloproteinase from the venom of Agkistrodon acutus from Guangxi.
目的:克隆桂北五步蛇金属蛋白酶原基因,并对其进行序列分析。
Aim: This study aims to screen the differentially expressed genes related to the pathogenesis of ameloblastoma by cDNA microarray.
目的:用生物芯片技术筛选成釉细胞瘤相关基因。
Using this improved method, we analyzed the gene expression patterns of H and SP, achieving 13 differentially expressed cDNA fragments.
应用该优化的方法,比较了H和SP的基因表达图谱,分离了13个差异表达的片段。
Conclusion These 2 specifically expressed cDNA fragments isolated by DD-PCR might be the colorectal carcinoma-related oncogenes in quest.
结论这2条差异显示新基因片段很可能是与大肠癌相关的致癌基因。
Objective to Screen the various expression of apoptosis related genes between glioblastomas and normal brain tissue with cDNA microarray.
目的研究胶质母细胞瘤凋亡相关基因的表达。
A watermelon mosaic virus 2 (WMV-2) isolate from diseased watermelon in Shanxi Province was used for cDNA synthesis of WMV-2 CP gene by RT-PCR.
本研究以从山西西瓜上分离纯化得到的西瓜花叶病毒2号(WMV-2)分离物为材料,用RT-PCR方法得到其外壳蛋白(CP)。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板,在消减文库构建时,能大大提高工作效率。
Objective: To construct and identify recombinant adeno-associated virus encoding rat tissue inhibitor of metalloproteinase-1(TIMP-1) full length cDNA.
目的:构建及鉴定载入金属蛋白酶组织抑制因子1(TIMP鄄1)的重组腺相关病毒载体。
Aim To investigate the effect of fibroblast growth factor 2 (FGF2) cDNA mediated by recombinant adeno-associated viral vector2 (rAAV-2) on ischemic myocardium.
探讨重组2型腺相关病毒载体介导2型成纤维细胞生长因子基因诱导家兔缺血心肌血管生成的作用。
Objective: to establish a stable cell line and employ the cell line as a model to study target integration of exogenous cDNA and modulation of gene expression.
摘要目的:建立稳定整合的细胞株,为研究外源基因定位整合机制及其对靶细胞生物特征影响,建立一个生物模型。
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