Supply anatomy data for repair of anterior cranial fossa injuries using calvaria and galea aponeurotica vescularized flaps.
提供使用颅盖和盖利腱膜囊状皮瓣修复颅前窝损伤的解剖学数据。
Methods The osteoblasts were isolated from human fetal calvaria.
方法体外分离培养人成骨细胞。
Osteoblasts were delievered and cultured from the new-born Wistar rats calvaria by the digesting method.
采用消化法从初生大鼠的颅盖骨中分离、培养成骨细胞。
Results The cells from rabbit calvaria were positive to ALP staining and could form calcium node in vitro.
结果从兔颅骨分离的细胞碱性磷酸酶染色阳性，在长期培养中能够形成钙化结节。
Methods: the osteoblasts obtained from the calvaria of neonatal SD rats were cultured by method of enzyme digestion in vitro.
方法：采用酶消化法传代培养乳sd大鼠颅盖骨细胞并建立缺氧模型。
Objective To supply anatomy data for repair of anterior cranial fossa injuries using calvaria and galea aponeurotica vescularized flaps.
目的为颅前窝组织缺损一次性修复重建提供研究资料。
Criteria for inclusion of an article required content related the mechanism of calvaria abnormalities, and unrelated and repetitive articles were excluded.
纳入与颅盖畸形形成机制有关的内容，排除不相关及重复文章。
Complications included pin loosening in 24, pin infection in 4, loss of the reduction in 4, penetration of inner plate of calvaria in 3 and pressure sores in 1 case.
并发症包括颅钉松动（24例）、钉孔感染（4例）、复位丢失（4例）、颅钉穿透颅骨内板（3例）、背心下皮肤压疮（1例）。
Materils and method: Obtaining experimental cellular model by isolating and culturing in vitro of osteoblasts from SD rats' fetal calvaria . Differentiation stages were divided .
材料与方法：用组织块培养方法作SD大鼠胎鼠骨源性成骨细胞的体外培养，获取实验细胞模型并划分其分化阶段。
Conclusion Further research about craniosynostosis and the growth factors involved will contribute to understanding the mechanism of calvaria abnormalities and help treat problems.
结论进一步研究颅缝过早闭合及相关生长因子的作用，将有助于阐明颅盖畸形形成机制，并更好地指导临床治疗。
Methods: the serum collected from experimental rats given soybean isoflavones extract was added to the culture system of osteoblast cells (ob) isolated from the calvaria of neonatal SD rats.
方法：以经口给予大鼠大豆异黄酮类提取物后分离的血清作为干预物，加入新生sd大鼠颅骨分离培养的成骨细胞(OB)中。
Methods: the serum collected from experimental rats given soybean isoflavones extract was added to the culture system of osteoblast cells (ob) isolated from the calvaria of neonatal SD rats.
方法：以经口给予大鼠大豆异黄酮类提取物后分离的血清作为干预物，加入新生sd大鼠颅骨分离培养的成骨细胞(OB)中。
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