Orthogonal design improved experimental efficiency and precision, which was a good way for selection and optimization of callus induction medium and differentiation medium.
正交设计可提高试验效率及准确性，是愈伤组织诱导培养基、分化培养基筛选优化的好方法；
With this medium, soft, transparent callus can be induced from immature embryos of embryo selection or tissue and organ differentiation stages. The induction frequency was 2-6%.
以该配方诱导幼胚，处于胚胎选择阶段和组织与器官分化阶段的幼胚，能诱导出松软、晶莹透明的愈伤，诱导频率为2-6％。
It was important for callus induction and shoot differentiation to choose better starting materials and a suitable medium in rice anther culture.
选择合适的水稻花培起始材料，选用适宜的培养基配方，对于水稻花培中愈伤组织诱导和分化至关重要。
Effects of NAA, 6-BA, MS culture medium on leaf and petiole callus induction of Changium smyrnioides Wolff were studied by orthogonal design.
采用正交设计方法，研究了NAA、6-BA、MS培养基对明党参叶片与叶柄愈伤组织诱导的影响。
Without added 2, 4-d in the medium, explant callus induction rate is very low, and needed a very long time to induct.
在不添加2,4 - D的培养基中，外植体的愈伤组织诱导率很低，并且需要诱导很长的时间。
With regard to the callus redifferentiation, not only kinetin is needed, but also the effect of auxin concentration of the induction medium is also remarkable.
对于愈伤组织的再分化，不仅需要细胞分裂素，而且诱导培养基中生长素浓度的影响也是显著的。
With young stem segment of Euonymus japonicus as explants, callus induction were studied on MS basal medium supplemented with different hormone combinations including BA and NAA, IBA , 2,4-D.
以大叶黄杨的幼茎段为外植体，在添加BA和NAA、IBA、2，4-D不同激素组合的MS培养基上培养，对其愈伤组织进行诱导试验。
Influence of different mass concentration of 2, 4-d on callus induction in maize inbred line 4112 from immature embryos with MS being used as basal culture medium was studied.
以玉米自交系4112的幼胚为外植体，以MS为基本培养基，研究了不同质量浓度的2,4 - D对愈伤组织诱导的影响。
M6 medium was suitable for growth of side-bud and callus Induction of subcutaneous tissue.
M6培养基适于侧芽生长和皮下组织诱导愈伤；
M6 medium was suitable for growth of side-bud and callus Induction of subcutaneous tissue.
M6培养基适于侧芽生长和皮下组织诱导愈伤；
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