Methods Human BMSCs were isolated, incubated and identified.
方法体外分离、培养和鉴定BMSC。
Histology characteristics of BMSCs were examined using toluidine blue staining.
骨髓基质细胞组织学检测采用甲苯胺蓝染色。
Results BMSCs could be effectively labeled and labelling efficiency were around 99%.
结果菲立磁可以高效率地标记骨髓基质细胞,标记效率在99%左右。
Cell surface marker staining confirms that the cells isolated in this study are BMSCs.
细胞表面标记染色鉴定证明,本实验所分离细胞是骨髓间充质干细胞。
Conclusion: Leptin can stimulate the differentiation of BMSCs on titanium surface in vitro.
结论:瘦素能够促进钛片表面骨髓间充质干细胞的分化。
MATERIALS: New Zealand big-eared rabbits aged 5 months were used for isolation and culture of BMSCs.
材料:5月龄新西兰大白耳兔用于骨髓间充质干细胞的分离培养。
Conclusion The transiently expression system of BMSCs modified by OPG gene is successfully established.
结论成功建立了OPG基因修饰的骨髓基质细胞瞬时基因表达体系。
The BMSCs have biocompatibility with CTCP, and CTCP could be used as vector for BMSCs in tissue engineering.
兔骨髓基质细胞与复合材料具有良好的生物相容性,可作为骨髓基质细胞的载体应用于组织工程。
OBJECTIVE: To explore effects of human BMSCs on t cell proliferation in patients with aplastic anemia in vitro.
目的:体外观察人骨髓间充质干细胞对再生障碍性贫血患者T细胞增殖的影响。
Results: BMSCs not only can enter the immunoorgans of allogeneic mouse, but also can exist there for at last 30 days.
结果:BMSC可进入并较长期(30天)存在于异基因小鼠免疫器官内;
The adhesion, growth, proliferation of BMSCs on the materials, as well as extracellular matrix secretion were observed.
观察细胞在生物材料上附着、生长、增殖及细胞分泌细胞外基质情况。
CONCLUSION: The compound composed of calcium alginate, BMSCs and growth factor might form bone and cartilaginous tissue.
结论:骨髓基质细胞-藻酸钙-生长因子复合材料能形成软骨及骨组织。
After measuring virus titer, BMSCs were transfected and the fluorescence intensity was observed under fluorescence microscope.
重组病毒物理滴度测定后感染骨髓基质干细胞,在荧光显微镜下观察荧光强度。
Human bone marrow mesenchymal sten cells (BMSCs) were seeded into CPC-PLGA polymer and cultured in vitro, then detected by SEM.
将人骨髓间充质干细胞种植于CPC - PLGA复合物上并体外培养,然后进行扫描电镜观察。
With the deep understanding of BMSCs differentiation into cartilage cells, the application of these factors will be more extensive.
随着骨髓间充质干细胞分化为软骨细胞的深入研究,这些因素的应用必将更为广泛。
Objective To investigate the effect of 5-azacytidine on cardiomyogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).
目的研究在体外5-氮胞苷诱导骨髓间质干细胞定向心肌向分化的条件。
Objective To search for a more efficient method to induce bone marrow stromal cells (BMSCs) into Schwann-liked cells (SLCs) in vitro.
目的探讨一种较为有效的诱导方法将骨髓基质干细胞体外定向诱导分化为类许旺细胞。
The viruses were used to infect directly BMSCs and the expression of BMP 7 gene in BMSCs was analyzed by immunohistochemical staining.
结果:成功地构建了重组BMP7逆转录病毒表达载体,并可在骨髓基质干细胞中表达BMP 7。
Objective:To investigate the possibility of induced differentiation of rat bone marrow stromal cells(BMSCs)into neural cells in vitro.
前言: 目的:探索骨髓基质细胞体外定向诱导分化为神经细胞的可能性。
In normal group, BMSCs with the same quantity were injected into epicardial sites in the left ventricle anterior wall by the same method.
正常移植组于左心室前壁心外膜下进行注射,方法及注射量同心肌梗死移植组。
CONCLUSION: BMSCs in combination with BMSC autologous mobilization have an improved effect on cardiac function in rats with heart failure.
结论:骨髓间充质细胞联合自体骨髓干细胞动员对心力衰竭大鼠心功能有明显改善作用。
Methods Rabbit BMSCs were cultured in vitro induced to osteoblasts, and then the biologic characters of the BMSCs was observed and tested.
方法体外培养获取生长状态良好的兔骨髓间充质干细胞,进行成骨诱导,观察并检测成骨细胞的生物学特性。
OBJECTIVE: To establish a method for in vitro isolation and cultivation of BMSCs from rabbits and to study their biological characteristics.
目的:拟建立一套体外分离培养及大量扩增兔骨髓间充质干细胞的方法,并对细胞生物学特性进行观察。
Conclusion Byuse complete marrow direct culture method and density gradient method, after 3 ~ 5 passages, relative purified BMSCs can be obtained.
结论采用全骨髓直接培养及密度梯度离心法,经3 ~5代培养后均可获得相对纯化之骨髓间充质干细胞。
Methods Rabbit BMSCs were cultured in vitro induced to chondrocytes, and then the biologic characters of the chondrocyte were observed and tested.
方法体外培养获取生长状态良好的兔骨髓间充质干细胞,进行成软骨诱导,观察并检测其生物学特性。
The effects of microgravity on the differentiation process from BMSCs to mature osteoblast and its possible mechanism were emphatically introduced.
着重介绍了微重力对骨髓间质干细胞到成熟成骨细胞整个分化过程的影响及其可能机制。
METHODS: The 3rd generation of BMSCs was randomly divided into blank group, control group and Chinese medicine with high, moderate and low dose groups.
方法:第3代骨髓间充质干细胞随机分为中药低剂量组、中药中剂量组、中药高剂量组、对照组和空白组。
OBJECTIVE: To observe the effect of bone mesenchymal stem cells (BMSCs) mixed with sodium hyaluronate solution in repair of disc degeneration in rabbits.
目的:观察透明质酸钠混合溶液复合骨髓间充质干细胞修复兔退变椎间盘的效果。
OBJECTIVE: To observe the effect of bone mesenchymal stem cells (BMSCs) mixed with sodium hyaluronate solution in repair of disc degeneration in rabbits.
目的:观察透明质酸钠混合溶液复合骨髓间充质干细胞修复兔退变椎间盘的效果。
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