By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
间接免疫荧光、ELISA和免疫印迹检测证明，含SO.7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
蛋白印迹实验鉴定转基因植株，ELISA方法测定重组蛋白表达水平，集落形成实验测定重组蛋白活性。
The results showed recombinant NP protein could bind to both positive serum and MAb with specificity in ELISA, but only bind to positive serum in western blot.
结果表明：所获得的重组NP蛋白在ELISA分析中均能与阳性血清和单克隆抗体发生特异性结合，但在免疫印迹分析中仅与阳性血清发生特异性结合。
Its immunoreactivity was analyzed by Western blot, ELISA and blocking ELISA using MAb and positive serum.
采用单克隆抗体和阳性血清经elisa、阻断elisa、免疫印迹分析重组蛋白的免疫反应性。
Methods HIV antibody were screened by ELISA and confirmed by western blot( WB)in 2 272 patients diagnosed with tuberculosis.
方法对2 272例诊断为结核病的住院病人进行HIV抗体检测，初筛阳性者进一步做免疫印迹试验（WB）。
Methods: the 233 drug users were surveyed using the same questionnaire, HIV antibodies were screened and confirmed using enzyme-linked immunosorbent assay (ELISA) and Western Blot (WB) respectively.
方法：采用同一问卷对强制戒毒所233名吸毒者进行调查，并用酶联免疫吸附试验(ELISA)和确认试验(WB)对吸毒者进行HIV初筛和确认。
Common technologies used for HCP analysis include ELISA (Enzyme-linked Immunosorbent Assay), one-dimensional or two-dimensional gel electrophoresis, Western Blot and Mass Spectrometry.
常见的用于宿主细胞蛋白的分析的技术包括酶联免疫吸附法(酶联免疫吸附试验)，一维或二维凝胶电泳，免疫印迹和质谱分析。
Common technologies used for HCP analysis include ELISA (Enzyme-linked Immunosorbent Assay), one-dimensional or two-dimensional gel electrophoresis, Western Blot and Mass Spectrometry.
常见的用于宿主细胞蛋白的分析的技术包括酶联免疫吸附法(酶联免疫吸附试验)，一维或二维凝胶电泳，免疫印迹和质谱分析。
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