Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
方法：采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本，通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
Method The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.
方法采用RT P CR技术、硫化pcr结合限制性内切酶技术检测白血病细胞系及正常人外周血单个核细胞WT 1基因的表达及其启动子区DNA甲基化水平。
To study dysfunction of activated T cell in peripheral blood and make clear its role on pathogenesis in patients with recurrent tonsillitis(RT).
目的探讨反复扁桃腺炎（RT）患儿T细胞活化功能障碍情况，为分析RT的病因和发病机制提供理论依据。
Methods Test blood donors for HCV-RNA by RT-PCR, and some positive cases were followed up.
方法用RT - P CR检测大批正常献血者的HCV - RNA，并对其中部分HCV - RNA阳性者做随访研究。
Objective To increase the sensitivity of quantitative detection of gene transcripts in peripheral blood using real time RT PCR, two specimen processing methods were compared.
目的选择合理的标本处理方法，提高外周血基因转录本检测的灵敏度。
Both medicine had no obvious effect to the hepatic function , renal function , blood-rt, urine-rt, stool-rt of all patients .
两组药物对肝、肾功能及血、尿、粪常规均无明显影响；
Methods: Eight spontaneous hypertensive rats (SHRs) and 8 WKYs (with normal blood pressure) were selected. The HO-1 mRNA expression in aortic smooth muscle cell was measured by rt PCR.
方法：选取自发性高血压大鼠(SH R)及与之相匹配的正常血压大鼠(WKY)各8只，应用rt- P CR技术检测主动脉血管平滑肌细胞血红素氧合酶基因表达。
Methods: Eight spontaneous hypertensive rats (SHRs) and 8 WKYs (with normal blood pressure) were selected. The HO-1 mRNA expression in aortic smooth muscle cell was measured by rt PCR.
方法：选取自发性高血压大鼠(SH R)及与之相匹配的正常血压大鼠(WKY)各8只，应用rt- P CR技术检测主动脉血管平滑肌细胞血红素氧合酶基因表达。
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