Methods: Hormone binding assay and gel mobility shift assay were used.
方法:用激素结合法和迁移率改变法。
Methods: radioligand binding assay andfunctional experiment of isolated perfused left atria were mesured.
方法:放射配体结合实验和离体左心房收缩功能实验。
Methods: receptor radioligand binding assay, receptor DNA binding assay and immunofluorescence methods were used.
方法:采用受体放射配基结合分析、受体-DNA结合分析及免疫荧光技术。
METHODS: The receptor binding assay with calf striatum to D1 and D2 receptors, and the animal behavior tests were used.
方法:用小牛纹状体膜蛋白对D_1和D_2受体进行放射受体结合分析并进行大鼠行为实验。
The activity of ADAMTS13 was measured by residue collagen binding assay, and antigen by a newly developed sandwich ELISA.
用新近建立的三抗体夹心酶联免疫反应法检测标本的ADAMTS13抗原含量。
Methods: Adopt some methods such as receptor binding assay, hot plate, the tail withdrawal reflex, EEG, hemodynamics and the randomized double blind evaluation.
方法:应用受体结合、热板、甩尾反射、EEG、血流动力学、随机双盲评价等方法。
Maximal binding volume of glucocorticoid receptor (GR) in hepatic tissue was assayed by radio-ligand binding assay and protein content was assayed by Western blot.
肝组织GR采用放射性配体结合分析法测定其最大结合容量以及用免疫印迹法测定其蛋白含量。
Using radioligand of binding assay of receptors determinates that betaine can inhibit the binding of EGF receptor with EGF and affect the TPK activity conspicuously.
应用受体的放射配基结合分析法测定出甜菜碱对大鼠肝细胞膜表皮生长因子受体(EGF)及其酪氨酸蛋白激酶(TPK)有显著的结合抑制作用。
The type and content of adrenoceptors in heart of normothermic and cold-acclimatized toad were studied by radioligand binding assay at different testing temperatures.
用放射配基结合分析法,研究了不同温度条件下蟾蜍心脏各部位肾上腺素能受体的类型和含量。
To determine whether melatonin receptors (MR) exist in human stomach, specific binding of melatonin receptor in human stomach was measured by radioligand binding assay.
为明确人体胃组织是否存在褪黑素受体(MR),应用放射配体结合法检测人体胃组织MR特异结合量。
The phosphorylation of STAT5 and its location were examined by double immunohistochemical staining and the binding of STAT5 with DNA was determined by electrophoretic mobility shift assay (EMSA).
用双染免疫组化法检查脾细胞中STAT 5磷酸化与STAT 5抗原的定位。用电泳迁移率变动分析(EMSA)测定STAT 5与DNA探针的结合力。
Dot blot assay was adopted to observe the binding of hemoglobin by P. nigrescens.
采用斑点印迹法观察黑色普氏菌能否与人血红蛋白结合。
To improve a gel retardation Assay for the study of binding in the nuclear protein and DNA.
目的:探讨改良的凝胶阻滞法用于核蛋白-DNA结合的检测。
Objective The specific property for DNA-binding of human telomerase was tested by gel mobility shift assay.
目的采用凝胶迁移阻滞法分析研究人类端粒酶dna结合特性。
First, we carried out Electrophoretic Mobility Shift Assay (EMSA) to investigate the binding difference of SNPs and transcription factors.
首先使用凝胶迁移检测和疾病关联的SNP是否会影响某些转录因子的结合。
The biotin binding and immunocytochemistry assay indicated that the expressed fusion protein was capable of binding biotin molecule and CEA antigen respectively.
活性鉴定表明该融合蛋白具有结合CEA及生物素的双特异性。
The functional expression of DNA binding domain of Zif268 will greatly facilitate the development of in vivo genetic selection assay for the study of Zinc fingers-DNA interaction.
锌指DNA结合区在大肠杆菌中的功能性表达成功为锌指蛋白DNA相互作用的胞内遗传筛选模型的建立奠定了基础。
The functional expression of DNA binding domain of Zif268 will greatly facilitate the development of in vivo genetic selection assay for the study of Zinc fingers-DNA interaction.
锌指DNA结合区在大肠杆菌中的功能性表达成功为锌指蛋白DNA相互作用的胞内遗传筛选模型的建立奠定了基础。
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