Influence of different mass concentration of 2, 4-d on callus induction in maize inbred line 4112 from immature embryos with MS being used as basal culture medium was studied.
以玉米自交系4112的幼胚为外植体,以MS为基本培养基,研究了不同质量浓度的2,4 - D对愈伤组织诱导的影响。
With young stem segment of Euonymus japonicus as explants, callus induction were studied on MS basal medium supplemented with different hormone combinations including BA and NAA, IBA , 2,4-D.
以大叶黄杨的幼茎段为外植体,在添加BA和NAA、IBA、2,4-D不同激素组合的MS培养基上培养,对其愈伤组织进行诱导试验。
W14 was the best basal media for the first step of callus induction.
W14 是适合花药愈伤组织诱导的基本培养基;
The experimental results show that when adopting MS basal medium, adding 2,4-D benefit to induce callus;
实验结果表明:以MS为基本培养基,在愈伤诱导培养基中加入2,4-D利于愈伤组织的诱导;
Callus formation frequency as well as plantlet regeneration frequency was dependent on the composition of basal medium and the concentration of 2, 4-d.
胚性愈伤诱导率和植株再生率与培养基的种类、培养基中2,4 - D的浓度、小麦基因型及诱导和分化培养基中激素种类和浓度等有关。
Callus formation frequency as well as plantlet regeneration frequency was dependent on the composition of basal medium and the concentration of 2, 4-d.
胚性愈伤诱导率和植株再生率与培养基的种类、培养基中2,4 - D的浓度、小麦基因型及诱导和分化培养基中激素种类和浓度等有关。
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