The gradient elution is reasonable when the retention time is 2.3 times after the appearance of prominent peak with initial mobile phase acetonitrile-water (40:60).
用初始流动相乙腈-水(40：6 0)洗脱至主峰出峰保留时间的2.3倍时开始进行梯度洗脱比较合理。
The gradient elution is reasonable when the retention time is 2.3 times after the appearance of prominent peak with initial mobile phase acetonitrile-water (40:60).
用初始流动相乙腈-水(40：6 0)洗脱至主峰出峰保留时间的2.3倍时开始进行梯度洗脱比较合理。
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