The reactions are typically carried out on a regulated heating block, or PCR machine, and consist of 30-35 cycles of repeated amplification of all the DNA present.
这一反应通常是在一可调控的温箱中或PCR仪中进行,对所有的DNA进行30到35个循环扩增。
With nanopores, however, virtually no chemical pre-processing is required and no amplification. Instead a single strand of DNA can be read, one base pair at a time, and without the need for labelling.
但是如果用纳米孔,实质上就不需要化学与处理和“放大”,取而代之的是,可以一次一个碱基对地读出单条dna链,也不用标记。
Sequencing technology has come a long way since the Human Genome Project, but it still requires DNA to be copied millions of times, a step called amplification, and labelled with fluorescent tags.
测序技术在人类基因组计划后有了长足的进展,但是它仍然要求DNA经过一项称为“放大”的步骤,被复制几百万次,然后再用荧光标记。
OBJECTIVE To establish the random amplification of polymorphic DNA (RAPD) typing technology for Proteus strains and apply it in the epidemiology investigation.
目的建立变形菌随机扩增dna多态性分型技术,应用于临床菌株流行病学调查。
A method for quickly preparing PCR amplification of dinoflagellate total DNA template was reported.
报道了一种快速制备P CR扩增甲藻细胞模板dna的方法。
CDK4 amplification could be correlated with HBV DNA integration and the progression of HCC.
CDK4的扩增可能与HBV -DNA的整合以及肝癌的进展有关。
Objective to research on the possibility and the efficiency of the enzyme biological signal amplification system applied to DNA piezoelectric biosensor.
目的研究“酶生物信号放大系统”用于放大dna压电传感器检测信号的可行性和有效性。
Genetic damage with DNA alterations leads to point mutations of genes, translocations of genetic material between chromosomes, and gene reduplication with amplification.
伴随DNA改变的基因损伤会导致基因的点突变、染色体间基因移位和扩增性基因复制。
DNA stock solution is based on customer autologous DNA as a template, after complete restoration, a lot of amplification, the re-import the customer's skin from the body of a DNA repair technology.
DNA原液是以客户自体dna为模板,经过完全的修复,大量的扩增之后,重新导入客户的皮肤的一种自体dna修复技术。
Validity oft his "non-DNA" principle was evaluated by amplification of globin gene from fresh tonsil tissues and plasmid gene on condition that gloves were contaminated by plasmid.
然后通过扩增新鲜扁桃体组织中珠蛋白基因及模拟手套被质粒污染的情况下扩增质粒基因,验证该无DNA操作过程对避免污染的有效性。
Rare amount of DNA sample often needs repeated amplification in order to remain enough amount of DNA template for various analysis.
基因操作者经常需要把数量稀少的DNA样品进行反复扩增以供进一步研究之用。
Random amplification of polymorphic DNA(RAPD)assay was used to study the finger-printing of 26strains from 16specie s.
以随机扩增DNA多态性法对16种支孢26株菌的DNA指纹图谱作了分析。
The method to extract genome DNA from old skeletal specimens of cetaceans was improved and the PCR amplification reaction was optimized.
改进了从鲸类陈旧骨骼标本中进行基因组dna提取和PCR扩增的方法。
Objective: To introduce a simple rapid exactive modified PCR for detection CMV DNA in amniotic fluid strand displacement amplification for prenatal diagnosis of congenital HCMV infections of fetus.
目的:介绍一种简便快速准确检测羊水中CMV-DNA的改良PCR-链置换式扩增用于诊断胎儿先天感染CMV。
The DNA amplification technique is used in genome sequencing, forensics and the diagnosis of various diseases.
这种DNA的放大技术被用于多种疾病的检验,诊断和基因组序列的测定。
Objective To study the fetal DNA in maternal plasma using multiplex PCR amplification of the short tandem repent (STR) systems.
目的采用短串联重复序列(STR)多态位点的复合扩增方法,研究孕妇血浆中胎儿DNA基因型。
The bio-bar-code amplification technology is a powerful nano analytical tool that can detect both proteins and DNA, as well as identify trace amounts of anthrax lethal factor and PSA.
生物条形码技术是一种功能强大的纳米分析工具,能够检测出蛋白质和DNA,也能够识别出微量的炭疽致命因子和PSA。
In contrast, another 7 fragments, which appeared only in the amplification of total DNA and never in that of cytoplasmic DNA, were thought amplified from nuclear DNA.
另有7个片段,在以胞质DNA为模板时未出现,而是仅仅出现在以总DNA为模板的扩增中,认为是来自核DNA 。
Methods Biopsy specimens of lymph nodes were obtained from 10 cases of lymphoma patients. The changes of DNA copies number (e. g. deletion and amplification) were detected using the CGH technique.
方法获取10例淋巴瘤患者的活检淋巴结组织,以CGH技术检测淋巴瘤基因组d NA拷贝数的变化。
Based on the GDNF gene sequence of human and chick, we cloned partial DNA sequences encoding mature protein of GDNF from genomic DNA of giant panda and crested ibis through PCR amplification.
本项研究经pcr引物设计后,以大熊猫和朱鹮的基因组d NA为模板,克隆了大熊猫和朱鹮的GDNF成熟蛋白基因。
After DNA elongation, such molecules can serve as a template for amplification and will be picked up in the final product, as will product generated by non-specific annealing of PCR primers.
颠末dna地断裂伸长率,这些分子能够作为模板扩增,将加速最终产物,如将产物所发生地非特异性退火地PCR引物。
After DNA elongation, such molecules can serve as a template for amplification and will be picked up in the final product, as will product generated by non-specific annealing of PCR primers.
颠末dna地断裂伸长率,这些分子能够作为模板扩增,将加速最终产物,如将产物所发生地非特异性退火地PCR引物。
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