One of the most advantageous and widely used method is the tandem affinity purification.
串联亲和纯化是目前应用最广泛、最具优势的方法之一。
OBJECTIVE To develop the bioaffinity ligands and affinity purification technology for human serum albumin.
目的研究人白蛋白特异的仿生亲和配体和仿生亲和分离技术。
Methods:The proteins which interacted with gankyrin were screened and analyzed by affinity purification technology and mass spectrometry.
目的用亲和纯化法纯化嗜水气单胞菌气溶素,从单因子水平分析嗜水气单胞菌的溶血特性。
Conclusion The modified MAC with ZBM is a much easier, time-saving and effective method for affinity purification of antibodies in ascites.
结论用尼龙滤膜zbm改进的膜亲和层析法,可用于纯化腹水中的单克隆抗体,且简便、省时、有效。
Immobilized metal ion affinity chromatography sorbents were prepared for purification of reduced glutathione (GSH) using chitosan as substrate.
制备了以壳聚糖为载体的金属螯合亲和色谱分离纯化还原型谷胱甘肽(GSH)。
Conclusion Dye affinity chromatography can be used as a refine purification method for purification of EDH and G-6-PDH from baker's yeast.
结论染料配基亲和层析可作为面包酵母乙醇脱氢酶和葡萄糖-6-磷酸脱氢酶的精制纯化方法。
Affinity membrane separation, a new technology, has advantages of both membrane separation and affinity chromatography, and is good for the separation and purification of biological products.
亲和膜分离技术是一项新兴的技术,兼有膜分离和亲和色谱的优点,可以有效地进行生物产品的分离和纯化。
Cu(II)-immobilized metal affinity chromatography (Cu(II)-IMAC), as a highly reliable analytical procedure, has been widely used in separation and purification of biomacromolecule.
铜离子固定金属亲和色谱作为一种有效的分析方法,已普遍应用于生物大分子的分离与纯化。
Methods a combination of euglobulin precipitation, ion-exchange chromatography, (NH4) 2so4 precipitation and affinity chromatography was used in the process of purification.
方法采用优球蛋白沉淀、离子交换色谱、(NH4)2so4沉淀和亲和色谱法进行纯化。
Affinity chromatography is a powerful tool used in the purification of antibodies. In this paper, different kinds of affinity ligands used in affinity chromatography are described.
亲和色谱是用于抗体纯化的有效方法,本文对亲和色谱配体的研究进展进行了综述。
Purpose To study purification method of the low molecular weight heparin(LMWH) by affinity chromatography.
目的探索亲和色谱纯化低分子肝素的方法。
Among all purification methods, affinity chromatography shows the excellent characteristics such as high selectivity, high yield, etc.
以亲和技术为基础的分离纯化方法与其它分离制备方法相比具有高选择性、高活力回收等优点。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
采用亲和层析和离子交换层析分离纯化,以细胞增殖实验测定表达蛋白的生物活性。
It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatography, and hydrophobic interaction chromatography.
进一步提供了可选择的额外的纯化步骤,包括溶胞产物过滤、阴离子交换层析、三联体亲和层析和疏水性相互作用层析。
So in this study, based on the mechanism of affinity chromatography, the affinity chromatography column with the heme immobilized was built to do purification of HBP.
根据亲和层析的原理,把血红素作为配基制备亲和层析柱用于纯化HBP,为进一步研究HBP打下基础。
Typically, in previous reports, the purification of CP mainly relied on the antibody, liquid chromatography and affinity tag-based strategies.
在先前的研究中,CP的分离主要依赖抗体、液相色谱以及亲合标签纯化等技术。
The affinity medium thus prepared has high bonded cellulose capacity and may be widely used in large-scale separation and purification of biological product and body's immunity treatment.
按这种方法制备的亲和介质提高了纤维素的键合容量,并将在生物产品的大规模分离纯化及人体免疫治疗等方面有广阔的应用前景。
The affinity medium thus prepared has high bonded cellulose capacity and may be widely used in large-scale separation and purification of biological product and body's immunity treatment.
按这种方法制备的亲和介质提高了纤维素的键合容量,并将在生物产品的大规模分离纯化及人体免疫治疗等方面有广阔的应用前景。
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