Affinity membrane separation, a new technology, has advantages of both membrane separation and affinity chromatography, and is good for the separation and purification of biological products.
亲和膜分离技术是一项新兴的技术,兼有膜分离和亲和色谱的优点,可以有效地进行生物产品的分离和纯化。
This is essentially the same as affinity chromatography.
这在本质上与亲和层析是一样的。
Both proteins were purified with Nickel chelate affinity chromatography.
经金属螯合层析纯化,获得了高纯度的表达蛋白。
Antibodies are purified by protein a and peptide affinity chromatography.
多克隆抗体采用蛋白a和肽亲和色谱法纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
抗体采用蛋白a和肽亲和层析法纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
抗体由蛋白a和肽段亲和层析技术纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
抗体由蛋白a和肽段亲和层析纯化得到。
The soluble 21 peptide was purified by using chitin affinity chromatography.
经几丁质亲和层析柱纯化21肽。
Serum pretreated with coupling affinity chromatography is better than membrane absorption.
亲和偶联预处理血清的效果好于膜吸收的效果。
Purpose To study purification method of the low molecular weight heparin(LMWH) by affinity chromatography.
目的探索亲和色谱纯化低分子肝素的方法。
PurposeThe aim is to purify human platelet factor 4 (PF4) using a monoclonal antibody affinity chromatography.
目的用单克隆抗体亲和色谱从人血小板破碎液中纯化血小板第4因子(PF4)。
The modified gene was expressed by IPTG induction and purified by affinity chromatography and cleavage in place.
IPTG诱导该基因的表达,亲和层析和原位裂解法纯化表达产物。
The GST-MBD4 fusion protein was purified from cell lysates using glutathione Sepharose 4b affinity chromatography.
用谷胱甘肽琼脂糖凝胶4b亲和介质从菌体裂解液中纯化了GST -MBD4融合蛋白。
The purity of inclusion bodies was above 70% and that of protein purified by nickel affinity chromatography was 95%.
提纯的包涵体纯度可达70%以上,用镍亲和层析方法纯化蛋白则可达到95%。
The present invention relates to new affinity chromatography mediums for cobra neurotoxin and its preparation method.
本发明涉及一类新的针对眼镜蛇神经毒素的亲和层析介质及其制备方法。
The monoclonal antibody and polyclonal antibody were respectively purified from ascites and serum by affinity chromatography.
亲和层析分别纯化腹水和血清得到单克隆抗体和多克隆抗体。
Heat shock protein 70 (HSP70) was purified from rat liver with DEAE ion exchange chromatography and ATP-affinity chromatography.
利用DEAE离子交换和atp亲和层析法从热休克的大鼠肝脏中分离纯化了热休克蛋白70 (HSP70)。
Methods Phosphoproteins were extracted from lysates of normal mice livers by phosphate metal affinity chromatography (PMAC) resin.
方法取正常小鼠肝脏,裂解肝脏后利用磷酸盐金属亲和层析(PMAC)树脂提取磷酸化蛋白。
They were further purified with DE-52 cellulose ion exchange chromatography and Dextran Blue-Sepharose 4B affinity chromatography.
该凝集素经乳糖尿素液抽提和离心分离后,再用DE-52纤维素柱和蓝色葡聚糖柱进一步纯化。
Results: The recombinant proteins were expressed successfully and the purity was over 90% after purified by affinity chromatography.
结果:经表达和纯化获得了纯度达90%以上的融合蛋白;
Among all purification methods, affinity chromatography shows the excellent characteristics such as high selectivity, high yield, etc.
以亲和技术为基础的分离纯化方法与其它分离制备方法相比具有高选择性、高活力回收等优点。
Diagnostic sensitivity and specificity of the DSA affinity chromatography of the liver-type ALP for primary hepatocarcinoma are all 100%.
用肝型碱性磷酸酶dsa强结合组分诊断原发性肝癌的灵敏度和特异度均可高达100%。
Conclusion Dye affinity chromatography can be used as a refine purification method for purification of EDH and G-6-PDH from baker's yeast.
结论染料配基亲和层析可作为面包酵母乙醇脱氢酶和葡萄糖-6-磷酸脱氢酶的精制纯化方法。
Immobilized metal ion affinity chromatography sorbents were prepared for purification of reduced glutathione (GSH) using chitosan as substrate.
制备了以壳聚糖为载体的金属螯合亲和色谱分离纯化还原型谷胱甘肽(GSH)。
The present invention also relates to the new method of using the new affinity chromatography mediums in purifying and producing cobra neurotoxin.
本发明还涉及用这类新的亲和层析介质纯化生产眼镜蛇神经毒素的新方法。
The recombinant protein was purified through Ni-chelating affinity chromatography, and the purity was above 90% explained by SDS-PAGE gel scan analysis.
利用镍柱亲和层析对表达的重组蛋白进行纯化,经SDS-PAGE及薄层扫描分析表明,重组蛋白的纯度在90%以上;
Antibodies are produced by immunizing animals with synthetic peptides, and are purified by combinations of Protein a and peptide affinity chromatography.
该抗体通过使用合成肽段免疫动物而获得,并经蛋白a和肽亲和层析纯化。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
表达产物用亲和层析一步法进行纯化,然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
Fusion protein expression was induced by IPTG. After renaturation, the protein was purified by affinity chromatography and the bioactivity was examined by ELISA.
IPTG诱导表达融合蛋白,包涵体蛋白经复性后亲和层析法纯化,ELISA方法测定蛋白活性。
In this study, a new method based on immobilized metal affinity chromatography (IMAC) for screening phage antibody library against antigen in solution was reported.
本研究报道一种基于固定化金属亲和层析(IMAC)的噬菌体抗体库液相筛选方法。
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