Objective To review research progress of adipose tissue derived stromal cells (ADSCs).
目的介绍近期国内外脂肪组织来源的基质细胞研究进展。
Objective To review research progress of adipose tissue derived stromal cells ( ADSCs ).
目的介绍近期国内外脂肪组织来源的基质细胞研究进展。
After integrated with ADSCs, cells migrated into the ACM and adhered to the surface of material and grew well.
复合脂肪源性干细胞后细胞向材料内部迁移，粘附，生长良好。
OBJECTIVE: to establish a method for the isolation and culture of human ADSCs and to explore the ability of osteogenic differentiation.
目的：探讨人脂肪源干细胞在体外分离培养扩增的方法及向成骨细胞诱导分化的能力。
OBJECTIVE: to explore the feasibility of inducing rat ADSCs into neuron-like cells with a new scheme and medium containing multiple factors.
目的：观察多因子联合诱导和分步诱导方案诱导大鼠脂肪组织源性干细胞体外分化为神经元样细胞的可行性。
RESULTS and CONCLUSION: ADSCs could be expanded steadily in vitro and flow cytometry revealed high expression of stem cell-associated markers.
结果与结论：体外分离培养的脂肪源干细胞生长稳定，扩增速度快。流式细胞仪检测结果显示其高表达干细胞相关抗原。
Based on the progress in this field, this review focuses on ADSCs' isolation, culture, biological characteristics and applications, as well as their research prospect.
本文综述了脂肪来源间充质干细胞的分离培养、生物学特性及其应用等进展，并对其研究前景进行了探讨。
Human ADSCs were isolated firstly in this study with improved method, through the exchange of the cell medium to remove the red blood cells, reducing the damage of cells.
本文首先用多次联合消化脂肪组织的方法分离培养脂肪干细胞，通过换液的方法去除红细胞，减小了红细胞裂解液对细胞的损伤。
SPIO-labeled ADSCs were transplanted into normal rat hearts and were in vivo imaged with MRI. Image findings on MRI were correlated with histological findings of the rat hearts.
将经过SPIO标记的干细胞移植入正常大鼠心脏，使用MRI进行活体成像观察并与病理结果进行对照分析。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养，保留贴壁细胞传代，分析脂肪间充质干细胞的纯度，观察细胞生长情况，检测其增殖活性、细胞周期。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养，保留贴壁细胞传代，分析脂肪间充质干细胞的纯度，观察细胞生长情况，检测其增殖活性、细胞周期。
应用推荐