The adherent cells were gained by cultivated in vitro.
体外培养以获得贴壁细胞。
The adherent cells approached confluence after 10 passages, and was increased in number about 1000-fold.
贴壁细胞传代培养10代后接近融合,细胞扩增了约10 0 0倍。
Results With phase contrast microscope, the rapidly adherent cells were uniformed small, large nucleus and epithelial-like.
透射电镜显示,胞质内细胞器稀少,细胞核大,核内具有丰富的异染色质;
The non-adherent cells of the cultures could generate CFU-GM for more than 6 weeks With a rapid decline in it's output as the cultures going on.
非贴壁层细胞形成CFU-GM的能力达6周以上,但随着培养时间的延长,CFU-GM的形成活性明显下降。
Scanning electron microscope indicated that adherent cells showed shuttle shape or multiple Angle shape, and the multiple apophysis showed irregular shape.
扫描电镜观察,黏附细胞为梭型或多角型表现,并有多个突起呈不规则形状。
The form of bone marrow MNC adherent cells was uniform and showed the shape of fibroblast, which was not found in UCB adherent cells but mixed with amounts of round shape cells.
骨髓mnc贴壁细胞形态均一,呈典型的成纤维细胞样,而脐血贴壁细胞未获得典型的成纤维细胞样细胞,混有大量的圆形细胞。
Artery and vein were removed and mesenchymal tissue was taken out. The primary cells were performed culture and purification with collagenase digestion method to obtain the adherent cells.
无菌条件下取正常足月剖腹产的脐带近胎儿段,剔除动静脉,取出其中的间质组织,胶原酶消化法原代培养细胞进行培养和纯化获得贴壁细胞。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养,保留贴壁细胞传代,分析脂肪间充质干细胞的纯度,观察细胞生长情况,检测其增殖活性、细胞周期。
Conclusions Ligustrazine can increase the expression of adherent molecule of bone marrow cells in immune mediated AA mice, thus promote the growth of hematopoietic cells.
结论川芎嗪能增强免疫介导再障小鼠骨髓造血细胞和基质细胞粘附分子的表达,有利于造血细胞的增生。
It is concluded there are hematopoitic adherent precursors in cord blood CD34+ cells and the HUCBAC shows some biological behavior of hematopoitic stromal cells.
结论:人脐血CD34+细胞群中存在造血基质细胞的前体细胞,人脐血贴壁细胞具有造血基质细胞的某些生物学特点。
Results Human MSCs were successfully isolated from UCB. These cells were adherent with a fibroblast like morphology.
结果从脐血中可以培养出msc,为成纤维细胞样的贴壁细胞。
There were cells adherent to the surface of materials as well as the inside of ventage in the cytoskeleton compound group.
细胞支架复合组材料表面及孔隙内均有细胞附着。
RESULTS AND CONCLUSION:PMSCs and BMSCs had the adherent growth ability, morphology of fibroblast-like cells and spindle-shaped appearance, but the latter had the slightly smaller size.
结果与结论:胎盘间充质干细胞、骨髓间充质干细胞均成贴壁生长、形态均一的成纤维样细胞梭形外观,但后者体积略小。
BM cells were cultured in medium containing autologous serum for 3 weeks to expand adherent MSC.
骨髓细胞在含有自体血清的培养基中培养3周为了扩增贴壁的间充质干细胞。
CD133-adherent tumor cells were trypsinized before collection for assays.
CD133粘附肿瘤细胞在进行检测前需要先采用胰蛋白酶处理。
CD133-adherent tumor cells were trypsinized before collection for assays.
CD133粘附肿瘤细胞在进行检测前需要先采用胰蛋白酶处理。
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