• The main applications of immobilized penicillin G acylase and lipase in industrial catalysis were introduced as well.

    同时介绍了固定化青霉素酰化酶固定化脂肪酶工业催化领域应用

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  • It was found that penicillin acylase activity of the immobilized cells was more stable than that of the intact cells.

    发现固定化细胞青霉素化酶天然细胞的稳定

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  • The genomic DNA was extracted from the selective cultures and the GL-7-ACA acylase gene was directly amplified by PCR technique.

    土壤的选择性培养液中提取微生物的DNA通过PCR的方法直接扩增成功得到GL-7-ACA酰化酶基因

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  • S-2-phenylpropionic acid was obtained by hydrolysis of 2-phenylpropionic ester catalyzed by penicillin G acylase (PGA) in the micro-aqueous phase.

    通过微水优化拆分制备S-2-苯基丙反应条件,确定青霉素拆分制备S-2-苯基丙酸的最佳工艺参数。

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  • Acylase I was immobilized on Y-type molecular sieve by using 4 kinds of methods, namely, adsorption, adsorption-crosslink, coupling and coupling-diazotization.

    Y型分子筛为载体,分别采用吸附、吸附-戊二醛交联法、联法和偶联-重氮法4方法对氨基化酶i进行固定化。

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  • Methods:The influence of ammonium sulfate and glucose on plasmid stability and penicillin G acylase activity were conducted in flask shakes and a 5L fermentor.

    发酵过程中维持低葡萄糖水平可以限制细胞的生长速率,提高质粒稳定性促进青霉素G化酶的合成。

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  • Conclusions the usage of the new immobilized penicillin acylase reactor in the 6-apa production will result in a higher yield of 6-apa and lower loss of immobilized penicillin acylase.

    结论6APA生产使用新的固定化青霉素化酶反应器提高6 APA收率减少固定化青霉素酰化酶的损耗

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  • Conclusions the usage of the new immobilized penicillin acylase reactor in the 6-apa production will result in a higher yield of 6-apa and lower loss of immobilized penicillin acylase.

    结论6APA生产使用新的固定化青霉素化酶反应器提高6 APA收率减少固定化青霉素酰化酶的损耗

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