The ABP1 and TIR1 prokaryotic expression vectors were transformed into expression host strain BL21 (DE3), then after, IPTG was added to induce expression.

将构建好的ABP1及TIR1原核表达载体，转化表达宿主菌bl21 (DE3)，加入诱导物iptg进行诱导表达。

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In this study, we found that expression form of ABP1 was soluble so we purified it in natural conditions, at the same time, the TIR1 was purified in inclusion body conditions.

针对两种蛋白的不同表达形式，ABP1蛋白采用天然条件下纯化的方式，而TIR1蛋白采用包涵体纯化的方式。

youdao

In this study, we found that expression form of ABP1 was soluble so we purified it in natural conditions, at the same time, the TIR1 was purified in inclusion body conditions.

针对两种蛋白的不同表达形式，ABP1蛋白采用天然条件下纯化的方式，而TIR1蛋白采用包涵体纯化的方式。

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