目的:确定瘦素及其功能受体在脱出间盘组织中的表达,阐明瘦素是否能促进鼠np细胞在体外的增殖。
Objective. To determine the expression of leptin and its functional receptor in herniated disc tissues, and to elucidate whether leptin can stimulate rat NP cells to proliferate in vitro.
正如MTT法和计数pcna阳性细胞比例所显示:瘦素的给予与鼠np细胞的增殖成剂量依赖性关系。
Leptin administration led to increased proliferation of rat NP cells in a dose-dependent manner, as shown by MTT assay and by counting the percentage of PCNA-positive cells.
正如MTT法和计数pcna阳性细胞比例所显示:瘦素的给予与鼠np细胞的增殖成剂量依赖性关系。
Leptin administration led to increased proliferation of rat NP cells in a dose-dependent manner, as shown by MTT assay and by counting the percentage of PCNA-positive cells.
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