目的构建人il -12的高效表达载体。
Objetive to construct human IL 12 efficient expression vector.
方法：采取目的基因改造及载体构建的方法，以期构建高效表达载体。
Methods: The method of modifying aim gene and constructing vector was adopted to develop a vector with high efficacy.
目的克隆人热休克蛋白70 (HSP70)基因，构建其原核高效表达载体。
Objective to clone human heat shock protein 70 (HSP70) gene for the construction of a prokaryotic expression vector.
另外，杆状病毒是非复制型载体，能高效表达目的蛋白，其作为基因转移载体在基因治疗中乙显示出良好的应用前景。
Additionally, baculovirus has been a new gene transfer vector used in gene therapy due to its advantages of the extremely high expression of foreign genes and being non-reproduce vector.
目的：构建缺氧诱导表达载体，以介导报告基因在缺氧环境下的特异、高效表达。
Objective: to construct hypoxia-inducible expression vector, which mediated reporter gene to express specially and efficiently in hypoxia environment.
结论：经酶切鉴定构建的人透明带蛋白3重组表达载体可高效表达重组人zp3蛋白。
Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein.
外源淀粉水解酶能在酵母中高效表达并分泌，其主要因素在于：在构建载体时组入强启动子；
The main factors for high efficiency expression and secretion of exogenous amylum hydrolase in S. cerevisiae are as follows: involvement of strong promoter in carrier construction;
随着基因治疗研究的不断深入，寻求一种高效、安全、靶向表达的载体已成为了基因治疗的核心问题之一。
With the deepening research of gene therapy, to find an efficient, safe, targeted expression gene vector had become one of the core issues of gene therapy.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
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