目的采用两种方法制备饲养层细胞，对比观察培养效果，求得最佳培养方法，以期实现其有效利用。
Objective Adopt two methods to produce cells of feeder layer and compare and observe its result of culture to find a better method of culture.
目的：为保持胚胎干细胞的高度未分化状态，饲养层细胞成为胚胎干细胞体外培养中不可缺少的条件。
AIM: Feeder layer cells are the necessary condition to prevent the differentiation of embryonic stem cells cultured in vitro.
目的寻求无血清、无饲养层细胞存在的情况下，胚胎干细胞向多巴胺能神经元定向诱导分化的最佳条件。
Objective to explore the optimal condition of direct differentiation into dopaminergic neurons of embryonic stem cells in serum-free and feeder layer cell free medium.
同时，比较了饲养层细胞类型、生长因子、胚胎发育阶段等因素对绵羊胚胎干细胞分离培养效果的影响。
The effect of feeder cell type, growth factors, and embryo developmental stage on establishment of sheep embryonic stem cell was also studied in present experiment.
结论采用无饲养层细胞培养体系构建自体角膜上皮组织，移植后可以成功修复角膜缘干细胞缺失的角膜上皮。
Conclusion the reconstructed rabbit corneal epithelium hi vitro with a free-feeding layer culture system could be autografted to repair total corneal limbal stem cells deficiency.
目的采用无饲养层细胞培养体系构建家兔角膜上皮组织，自体移植后观察其对角膜缘干细胞缺失的治疗效果。
Objective to reconstruct rabbit corneal epithelium in vitro with a free-feeding layer culture system and autograft to treat limbal stem cells deficiency.
为维持细胞在培养过程中的未分化状态，需要采用饲养层细胞培养，同时设计合理的培养液配方并添加多种抑制分化或促进增殖的细胞因子。
Feeder layer cells and suitable culture medium that supplemented with cytokines of promoting proliferation or restraining differentiation were crucial to keep chicken es in an undifferentiated state.
从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
目的以原代培养小鼠骨髓基质细胞为饲养层，探讨精原干细胞能否在该饲养层上生长及生长的影响因素。
To study whether spermatogonial stem cells can grow on the feeder layers of bone marrow stromal cells, and their effecting factors in vitro.
详细系统地说明了迄今为止建立人胚胎干细胞系所需要的饲养层类型、培养基组成、添加细胞因子种类及其作用；
Selection of the feeder layer, medium, and supplemental cytokines and their roles in establishing and maintaining human ES and EG cell lines at present are illustrated in detail systematically.
在ES细胞培养过程中，有饲养层体系优于无饲养层体系。
Feeder system was superior to feeder-free system during the culture of ES cells.
观察鼠囊胚在小鼠原代胎儿成纤维细胞饲养层上的生长行为。
To observe the mouse blastocyst growth behavior on primary mouse embryonic fibroblast feeder layers (PMEF).
目的建立人胚胎干细胞饲养层培养体系。
Objective To establish the culture system of human embryonic stem-like cells feed layer.
人胚胎成纤维细胞饲养层在ES细胞分离过程中具有较好的作用。
Human embryonic fibroblast feeder played a positive role during the ES cells isolation procedure.
目的：建立ICR小鼠胚胎成纤维细胞饲养层培养体系，用于胚胎干细胞建系及研究。
Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast (MEF) cells for isolation and culturing of embryonic stem (es) cells.
结论：在复苏后早期阶段ES细胞培养中，不宜单独应用BRL条件培养基，须用MEF饲养层或合并应用BRL条件培养基和MEF饲养层。
Conclusion: ES cells should be cultivated on MEF feeder layer or in BRL conditioned medium plus MEF feeder layer at the early stage after recovering.
结果分别获得了传16代的胎鼠成纤维细胞和传7代的人胎儿成纤维细胞，建立了人胚胎干细胞饲养层的培养体系。
Results Acquired 16 passages in mouse and 7 passages in human and established the culture system of human embryonic stem-like cells.
因此，在没有饲养层的条件下，表皮干细胞的培养必须添加条件培养基。
Thus, the serum medium can't be used as ESCs medium without feeder.
撤除饲养层，观察人胚胎干细胞体外分化情况。
Without feeder layer, human embryonic stem cell differentiation was observed in vitro.
目的探讨建立有效的小鼠胚胎成纤维细胞饲养层，用于胚胎干细胞的培养。
Objective To study the isolation and culture and application of Kunming mouse embryonic fibroblasts(MEF).
在饲养细胞层和多种生长因子的共同作用下，可保持原生殖细胞在体外不断增殖而不分化，最终建立EG细胞系。
When cultured on feeder cells in the presence of many kinds of cytokines, primordial germ cells can proliferate continually and maintain undifferentiated state.
在饲养细胞层和多种生长因子的共同作用下，可保持原生殖细胞在体外不断增殖而不分化，最终建立EG细胞系。
When cultured on feeder cells in the presence of many kinds of cytokines, primordial germ cells can proliferate continually and maintain undifferentiated state.
应用推荐