经本文所描述的方法得到的DNA,完全能满足随机扩增多态性DNA(RAPD)分析。
The DNA drown by the method in this paper could completely suitable for RAPD analysis.
目的探讨白色念珠菌磷脂酶活性与随机扩增多态性DNA(RAPD)电泳条带之间的关系,构建多元回归模型。
Objective To research the relationship between the phospholipase activities and the random amplified polymorphic DNA(RAPD) bands of Candida albicans, and establish the regression model.
结果筛选出的33个随机引物对68个柞蚕品种(系)共扩增出296条DNA带,其中多态性带269条(90.88%)。
ResultA total of 296 DNA fragments were amplified with 33 primers selected from 68 cultivars of A. pernyi, 269 of which (90.88%) were polymorphic.
应用随机扩增DNA多态性分析技术(RAPD)分析产金属酶菌株的同源性。
Random amplified polymorphic DNA(RAPD) typing was carried out to analyze the homology of the isolates.
以随机扩增DNA多态性法对16种支孢26株菌的DNA指纹图谱作了分析。
Random amplification of polymorphic DNA(RAPD)assay was used to study the finger-printing of 26strains from 16specie s.
目的应用随机扩增DNA多态性(RAPD)技术对医院感染的粘质沙雷菌进行基因分型。
Objective To evaluate the genetic polymorphism of Serratia marcescens by randomly amplified polymorphic DNA (RAPD) method.
目的筛选出反应体系的最佳优化方案用于绿脓假单胞菌的随机扩增多态性DNA分析(RAPD)。
Objective To obtain optimum scheme on reaction system for randomly amplified polymorphic DNA(RAPD) of Pseudomonas aeruginosa.
所分析的20个随机引物共扩增出了109条带,但是只有引物s303、S4出现4个多态位点,多态性位点所占的比例(P)只有3.7%。
Among 109 loci revealed by 20 RAPD primers, only primer S303 and S4 showed 4 polymorphic loci. The percent of polymorphic loci (p) was only 3.7%.
目的建立随机扩增多态性DNA技术(RAPD)对甲氧西林耐药溶血性葡萄球菌(MRSH)的分型方法。
Objective To establish the Random Amplified Polymorphic DNA (RAPD) Technique for typing methicillin resistant Staphylococcus haemolyticus (MRSH).
目的探索随机扩增多态性DNA(RAPD)技术对大陆光亮钉螺遗传多态性研究的可行性,以获得不同地区光壳钉螺基因组DNA水平上的差异信息。
Objective Using RAPD technique to study genetic diversity of smooth-shelled Oncomelania hu- pensis in China and to gain more information on genome DNA level.
方法用随机扩增多态性DNA分析技术对恶性肿瘤患者深部真菌感染的主要病原菌基因进行分析。
Methods the genes of the main pathogen were analyzed by using Randomly Amplified Polymorphic DNA technique.
应用随机扩增多态性DNA(RAPD)标记,对东北地区不同狍种群的遗传变异进行了研究。
The random amplified polymorphic DNA(RAPD) markers were used to analyze genetic diversity among 3 geographical populations of the roe deer(Capreolus capreolus) in northeastern China.
应用23个随机引物对白粉病菌8个不同生理小种进行RAPD分析,其中11个引物的扩增带型在不同生理小种间出现差异,共扩增出59条谱带,多态性为47.5%。
Differences were found among 8 strains from RAPD analysis. Out of 23 random primers, 11 were polymorphic. Altogether 59 RAPD bands were found and the polymorphic rate was 47.5%.
在51条随机引物中筛选出具有稳定扩增、条带清晰的多态性引物16条,16条引物共检测到132个位点,其中多态位点114个(86.36%)。
RAPD primers were synthesized and 16 primers were selected and used. A total of 132 loci were amplified, of which 114 loci(86.36%) were polymorphic.
利用随机引物对基因组DNA扩增结果表明,NIL -PL0 1和NIL -APL0 1之间多态性差异较小,遗传同质性达98.6 % ,该材料正用于筛选与无花瓣性状紧密连锁的分子标记。
The genomic DNA PCR indicated that the genetic homogeneity between NIL-PL01 and NIL-APL01 reached 98.6%, which were being used in screening the molecular marker linked to apetalous trait.
利用随机引物对基因组DNA扩增结果表明,NIL -PL0 1和NIL -APL0 1之间多态性差异较小,遗传同质性达98.6 % ,该材料正用于筛选与无花瓣性状紧密连锁的分子标记。
The genomic DNA PCR indicated that the genetic homogeneity between NIL-PL01 and NIL-APL01 reached 98.6%, which were being used in screening the molecular marker linked to apetalous trait.
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