目的筛选出最佳随机引物并优化其反应体系用于8种细菌的随机引物聚合酶链反应（AP PCR）分析。
OBJECTIVE To screen the primers and optimize the reaction systems for arbitrarily primed polymerase chain reaction(AP-PCR) on 8 sorts of bacteria.
方法对医院临床分离的8株耐亚胺培南铜绿假单胞菌进行随机引物聚合酶链反应（PCR）扩增，扩增产物进行电泳和聚类分析。
METHODS The genes of imipenem-resistant P. aeruginosa were amplified by RAPD assay in 8 clinical isolates and PCR products were analyzed by agarose gel electrophoresis and cluster analysis.
方法对医院临床分离的8株耐亚胺培南铜绿假单胞菌进行随机引物聚合酶链反应（PCR）扩增，扩增产物进行电泳和聚类分析。
METHODS The genes of imipenem-resistant P. aeruginosa were amplified by RAPD assay in 8 clinical isolates and PCR products were analyzed by agarose gel electrophoresis and cluster analysis.
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