目的观察标记引物法、随机渗入标记法、末端转移标记法三种荧光标记法对寡核苷酸芯片杂交信号的影响。
Objective to observe the impact of three fluorescence marker methods of primer marker, random inleakage marker and terminal transfer marker to Oligonucleotide microarray hybridize signal.
对双随机引物的RAPD标记的效果进行了分析。
利用随机引物对基因组DNA扩增结果表明，NIL -PL0 1和NIL -APL0 1之间多态性差异较小，遗传同质性达98.6 % ，该材料正用于筛选与无花瓣性状紧密连锁的分子标记。
The genomic DNA PCR indicated that the genetic homogeneity between NIL-PL01 and NIL-APL01 reached 98.6%, which were being used in screening the molecular marker linked to apetalous trait.
运用22个随机引物扩增供试菌株得到151个RAPD标记，其中多态性标记占66.9%。
Of 151 RAPD bands that were amplified with 22 random primers and 66. 996 were polymorphic.
运用22个随机引物扩增供试菌株得到151个RAPD标记，其中多态性标记占66.9%。
Of 151 RAPD bands that were amplified with 22 random primers and 66. 996 were polymorphic.
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