突变位点经限制性内切酶分析证实。
Restriction enzyme site analysis was used to confirm the mutation.
结果表明，限制性内切酶的表达形式十分混乱。
The results reveal that its expression is rather disordered.
经限制性内切酶反应，获得两个预期大小的片段。
After a restriction endonuclease cleavage, two expected smaller fragments were observed.
PCR产物经限制性内切酶消化、序列测定及分析。
最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。
Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the AGAR gel electrophoresis.
首先用限制性内切酶将大的DNA分子切断，产生出特殊的片段。
Large DNA molecules are first dissected with restriction enzymes to produce specific fragments.
建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
根据揭示多态性的限制性内切酶的数量可将产生的突变大多归为点突变。
Based on the number of restriction enzyme detecting RFLPs, most of mutations were attributed to point mutation.
细菌有一种对病毒的防御机制：一种叫做限制性内切酶的化学物质可以切断外源dna。
Bacteria have defences against viruses in the form of chemicals called restriction enzymes, which chop up foreign DNA.
结果显示不论DNA甲基化与否，所有使用受体细胞限制性内切酶的基因组移植都获得成功。
When genome transplantations were performed using the restriction enzyme minus recipient cells, all the genome transplantations worked regardless of if the DNA was methylated or not.
结果表明，CE的分离效能明显高于age，是研究d NA限制性内切酶谱的更有效的检测手段。
Compared with AGE, CE is more outstanding in resolution and detection time, and it can be applied as a more effective means for DNA restriction enzyme pattern analysis.
方法对116例随机收集的1型糖尿病患者用聚合酶链反应-限制性内切酶消化作该点突变的筛选；
Methods The mutation had been screened from 116 unrelated patients with type 1 diabetes mellitus by ApaI digestion of product of polymerase chain reaction amplification.
锌指核酸酶是一种人工制做限制性内切酶，通过将锌指DNA结合区与限制性内切酶的DNA切割区融合获得。
Zinc finger nucleases (ZFNs) are artificial restriction enzymes made by fusing an engineered zinc finger DNA-binding domain to the DNA cleavage domain of a restriction enzyme.
这种技术可在DNA靶标分子的任意位点进行基因敲除、敲入、点突变等操作，无需使用限制性内切酶和连接酶。
The Red mediated recombination can be used to insert, delete or substitute DNA sequences at any desired position on a target molecule without the need for restriction enzymes or DNA ligases.
方法：采用PCR技术和限制性内切酶片段长度多态性（RFLP）的方法，检测中国北方汉族168个核心家系的基因型。
Methods A PCR-based RFLP procedure was employed to detect the genotypes of 168 family trios of Han descent population in the North of China.
方法：运用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR -RFLP)检测MTHFR的677位点多态性。
Methods: PCR-RFLP technique was used for detecting the A677V polymorphism site of MTHFR gene.
该方法应用荧光显微术使单个DNA分子成像，这些单个的DNA分子已经被分成有序的片断，因而被称为限制性内切酶的识别序列。
The method USES fluorescence microscopy to image individual DNA molecules that have been divided into orderly fragments by so-called restriction enzymes.
对36个水稻骨干系进行了RFLP（限制性内切酶长度多态性）分析，并对这些水稻材料的162个杂交组合育性进行了分组分类研究。
RFLP analysis was carried out on 36 rice elite line. Sterility of these 162 rice hybrids was studied in groups and in types.
以甘蓝型油菜的28个基因组探针和两种限制性内切酶对包括46个中国品种、9个欧洲品种在内的59个甘蓝型油菜品种(系)的RFLP标记进行了分析。
RFLP patterns were analyzed from 59 cultivars of Brassica napus, including 46 Chinese and 9 European accessions, using 28 genomic probes of Brassica and 2 restriction enzymes.
方法：采用PCRSSCP法检测血管网织细胞瘤中VHL基因的突变率及甲基化，敏感限制性内切酶消化法检测血管网织细胞瘤中VHL基因的异常甲基化率。
Methods: The hypermethylation was examined by methyl sensitive restrictive DNA endoenzyme analysis in 34 cases of angioreticuloma and the VHL gene mutations detected by PCR SSCP analysis.
方法采用RT P CR技术、硫化pcr结合限制性内切酶技术检测白血病细胞系及正常人外周血单个核细胞WT 1基因的表达及其启动子区DNA甲基化水平。
Method The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.
方法采用RT P CR技术、硫化pcr结合限制性内切酶技术检测白血病细胞系及正常人外周血单个核细胞WT 1基因的表达及其启动子区DNA甲基化水平。
Method The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.
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