方法：采用PCR技术和限制性内切酶片段长度多态性（RFLP）的方法，检测中国北方汉族168个核心家系的基因型。
Methods A PCR-based RFLP procedure was employed to detect the genotypes of 168 family trios of Han descent population in the North of China.
方法：运用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR -RFLP)检测MTHFR的677位点多态性。
Methods: PCR-RFLP technique was used for detecting the A677V polymorphism site of MTHFR gene.
首先用限制性内切酶将大的DNA分子切断，产生出特殊的片段。
Large DNA molecules are first dissected with restriction enzymes to produce specific fragments.
经限制性内切酶反应，获得两个预期大小的片段。
After a restriction endonuclease cleavage, two expected smaller fragments were observed.
经限制性内切酶反应，获得两个预期大小的片段。
After a restriction endonuclease cleavage, two expected smaller fragments were observed.
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